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What is a sense probe in insitu hybridisation?
A sense probe in in situ hybridization is a nucleotide sequence that is complementary to the target RNA or DNA sequence of interest, allowing for the detection of specific genes within tissue samples. Unlike antisense probes, which bind to the target sequence to visualize gene expression, sense probes bind to the non-template strand and serve as a control to confirm the specificity of the hybridization signal. The presence of a signal with a sense probe typically indicates non-specific binding, while a lack of signal supports the specificity of the antisense probe results.
Which of the following determines the specificity of a DNA probe?
complementary base pairing-apex
What is a tag or probe?
A tag or probe is a molecular tool used in various biological and chemical assays to detect or quantify specific substances, such as proteins, nucleic acids, or small molecules. Tags are often attached to antibodies, nucleotides, or other biomolecules, enabling visualization or measurement through techniques like fluorescence, radioactivity, or enzymatic activity. Probes can be either specific sequences of nucleotides that bind to complementary DNA or RNA or small molecules that bind to target proteins. Overall, tags and probes are essential for studying biological interactions and processes.
Can The activation site of an enzyme bind to a specific subtrate?
The activation site of an enzyme can only bind to a specific substrate.
Why is the hybridization probe said to be complementary?
The hybridization probe is said to be complementary because it is designed to bind specifically to its complementary target sequence in the DNA or RNA of interest. This complementary binding is necessary for the probe to accurately detect and identify the target sequence in a sample.
What is the protein handle on a chromosome?
The protein handle refers to specific regions on a chromosome where proteins can bind and interact with DNA. These protein handles help regulate various cellular processes such as gene expression, DNA replication, and chromosome structure. They play a crucial role in organizing and controlling the functions of the chromosome.
Region where enzyme and subtrate bind?
The active site is the region where the enzyme and substrate bind together. It is a specific location on the enzyme where the substrate molecules can fit and undergo a chemical reaction. The active site is crucial for the enzyme to catalyze the reaction efficiently.
What does formamide do in hybridization buffers?
Formamide lowers the melting point of nucleic acids so that the strands separate more readily. DNA is normally more stable in a double-stranded structure (even if every base isn't complementary) and less stable when single-stranded, so formamide must increase the stability of single-strandedness. In in situ hybridization, an RNA probe binds to mRNA that is already single-stranded. mRNA does not gain any stability by being a hybrid unless the probe is specific and can bind properly, thus increasing stability. For example, in the presence of formamide, a U nucleotide would rather bind to an A than nothing (binding to specific probe is better than staying single stranded), but a U nucleotide would rather bind to nothing than a G (binding to non specific probe is worse than binding to nothing). https://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_33-37.pdf
What specific sequences do transcription factors bind to on DNA?
Transcription factors bind to specific DNA sequences called enhancers and promoters to regulate gene expression.
Does ATP bind to an enzyme at more than one place?
cellular proteins have an specific site where ATP can bind
How many types of antigens can bind to a single antibody?
An antibody can typically bind to one specific type of antigen.
How do restriction enzymes determine the specific location to cleave DNA molecules during the process of genetic modification?
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules called recognition sites. These enzymes bind to these sites and cleave the DNA at specific locations, allowing for precise genetic modification.
