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Which of the following determines the specificity of a DNA probe?
complementary base pairing-apex
Can The activation site of an enzyme bind to a specific subtrate?
The activation site of an enzyme can only bind to a specific substrate.
What is the protein handle on a chromosome?
The protein handle refers to specific regions on a chromosome where proteins can bind and interact with DNA. These protein handles help regulate various cellular processes such as gene expression, DNA replication, and chromosome structure. They play a crucial role in organizing and controlling the functions of the chromosome.
Why is the hybridization probe said to be complementary?
The hybridization probe is said to be complementary because it is designed to bind specifically to its complementary target sequence in the DNA or RNA of interest. This complementary binding is necessary for the probe to accurately detect and identify the target sequence in a sample.
Region where enzyme and subtrate bind?
The active site is the region where the enzyme and substrate bind together. It is a specific location on the enzyme where the substrate molecules can fit and undergo a chemical reaction. The active site is crucial for the enzyme to catalyze the reaction efficiently.
What does formamide do in hybridization buffers?
Formamide lowers the melting point of nucleic acids so that the strands separate more readily. DNA is normally more stable in a double-stranded structure (even if every base isn't complementary) and less stable when single-stranded, so formamide must increase the stability of single-strandedness. In in situ hybridization, an RNA probe binds to mRNA that is already single-stranded. mRNA does not gain any stability by being a hybrid unless the probe is specific and can bind properly, thus increasing stability. For example, in the presence of formamide, a U nucleotide would rather bind to an A than nothing (binding to specific probe is better than staying single stranded), but a U nucleotide would rather bind to nothing than a G (binding to non specific probe is worse than binding to nothing). https://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_33-37.pdf
What specific sequences do transcription factors bind to on DNA?
Transcription factors bind to specific DNA sequences called enhancers and promoters to regulate gene expression.
Does ATP bind to an enzyme at more than one place?
cellular proteins have an specific site where ATP can bind
How many types of antigens can bind to a single antibody?
An antibody can typically bind to one specific type of antigen.
How do restriction enzymes determine the specific location to cleave DNA molecules during the process of genetic modification?
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules called recognition sites. These enzymes bind to these sites and cleave the DNA at specific locations, allowing for precise genetic modification.
Where do enhancers bind within the cell to regulate gene expression?
Enhancers bind to specific regions of DNA within the cell to regulate gene expression.
Do activators bind to enhancers to regulate gene expression?
Yes, activators bind to enhancers to regulate gene expression by increasing the transcription of specific genes.
