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The pKA of enzyme affects its ionization which could alter enzyme activity. For pH < pKa, the value of vmax is constant and that for pH > pKa, vmax decreases; ie. enzyme activity starts to decline.
Three things that can alter the rate of an enzyme are; temperature, pH and substrate concentration. Enzymes will have an optimal temperature and pH, at which they will have the greatest rate. Below or above these optimum conditions, the rate will be slower.
All enzymes have a range of pH where they are most effective. Too acidic or too basic environments will cause the enzyme to decrease in effectiveness, potentially stopping the enzyme from functioning all together.
1. Temperature. 2. P.H level. 3. Enzyme concentration
pH and temperature.
The pKA of enzyme affects its ionization which could alter enzyme activity. For pH < pKa, the value of vmax is constant and that for pH > pKa, vmax decreases; ie. enzyme activity starts to decline.
If you change the pH, the enzyme can become inactive and won't be able anymore to forfill its function.
Three things that can alter the rate of an enzyme are; temperature, pH and substrate concentration. Enzymes will have an optimal temperature and pH, at which they will have the greatest rate. Below or above these optimum conditions, the rate will be slower.
Enzymes work best at the optimum pH of 7.0. The addition of sulfuric acid causes the pH to become lower and this deactivating the enzyme. This change in pH can cause the side chains of an amino acid to change in its charge which can result in a change of protein. This can greatly alter the reaction that was to take place.
All enzymes have a range of pH where they are most effective. Too acidic or too basic environments will cause the enzyme to decrease in effectiveness, potentially stopping the enzyme from functioning all together.
A change in pH can denature an enzyme, meaning the reaction would stop.
Enzymes lower the amount of Activation Energy needed for a chemical reaction, therefore speeding up the chemical reaction. For an enzyme to do this it needs to be at the correct pH, salinity, and temperature otherwise the enzyme will not be able to work. When an enzyme is in a pH that is not suitable, the enzyme's shape and structure alter and make it unable to speed up a reaction.
1. Temperature. 2. P.H level. 3. Enzyme concentration
The enzyme with lowest pH optimum is pepsin. Pepsin works in the stomach in an acidic environment and aids in digestion.
A change in pH or and change in temperature will change the shape of the enzyme because its proteins are denatured. Therefore, the enzyme can no longer perform its desired functions because its specific shape has been altered.
pH and temperature.
Temperature, pH, organic solvent, mechanical forces