Want this question answered?
to differentiate between the lactose fermented bacteria or non- lactose fermented bacteria
Lactose
Levine eosin methylene blue agar does not contain lactose. It is a selective and differential medium used for the isolation and differentiation of gram-negative enteric bacteria based on their ability to ferment lactose. The agar contains lactose as a substrate for fermentation, but the actual quantity of lactose present in the agar may vary depending on the manufacturer and the specific formulation of the medium.
Milk sugar is Lactose. A table sugar is medium sized granulated.
It is always an unnamed substance that u can call anything
No, the pink means that the organism can ferment lactose, so the medium is acidic that why the top is pink. But the brown means the the organism can't ferment lactose and it hydrolyzed protein to amino acids. This is contradictory it's either the whole this is completely pink (lactose fermentation) or the medium is pink at top and rest is white (lactose and acid) or the top is purple and the rest of medium is brown (proteolysis/peptonization)= no lactic acid fermentation, so breaks down proteins to amino acids.
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
a medium
me
Straw!,
Lactose is not a macromolecule. It is a disaccharide, that is one molecule of lactose is formed by the elimination of water between two simple sugar molecules, in this case galactose and glucose. Lactose can be made to link up into medium chain molecules, but this is a fairly random process and produces a mixture of products which are not digestible.
Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue