Details: Hey guys. So I conducted an catalase activity experiment at school.We measured the speed of catalase activity on the disassembling of H2O2 solution. The independent variable was pH level, and the levels were 4, 6, and 10. The internet says that the catalase works best pH 7, but ironically, the catalase worked the best at pH10 in my experiment. Now I have to write Claim-Evidence-Reasoning, but I can't think of an appropriate reasoning because the result was different from what is on the internet. Can anyone explain why the catalase worked best at pH 10?
Catalase Hydrogen peroxide -----------------> Water + Oxygen.
Catalase work optimal in pH 7. Enzyme work specificly on certain pH. If change in pH occurs, it will slowing down the reaction rate. If the solution is too acidic (low pH value) or too basic (high pH value) the catalase is inactive - no longer functions as an enzyme. So that's why your cells should be neutral (pH 7). Our bodies are creating Hydrogen peroxide during normal metabolic events and catalase is present in the peroxisomes of nearly all human cells. There, it serves to protect the cell from any toxic effects by catalyzing the decompostion of H2O2 without the production of Oxygen free radicals. Catalase takes two molecules of hydrogen peroxide and converts them to two water molecules plus a molecule of oxygen gas. Hydrogen peroxide is a toxic molecule. Amount of solution in test tube is called the controlled variable. Controlled variables must be carefully monitored and kept equal in your experiments - otherwise they could mess up your experiment by making your results false or unreliable.
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The optimum pH for human catalase is approximately 7, and has a fairly broad maximum (the rate of reaction does not change appreciably at pHs between 6.8 and 7.5). It doesn't work as well above 7.5 or below 6.8.
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Catalase is an enzyme and enzymes work best with a specific substrate. The enzyme can work with any substrate just not as efficient .
between 35 and 40 degrees
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Catalase Hydrogen peroxide -----------------> Water + Oxygen.
it works the best at 37 degrees then after that it stops working .it doesn't work when it gets too cold. im doing an experiment on it now!
Catalase work optimal in pH 7. Enzyme work specificly on certain pH. If change in pH occurs, it will slowing down the reaction rate. If the solution is too acidic (low pH value) or too basic (high pH value) the catalase is inactive - no longer functions as an enzyme. So that's why your cells should be neutral (pH 7). Our bodies are creating Hydrogen peroxide during normal metabolic events and catalase is present in the peroxisomes of nearly all human cells. There, it serves to protect the cell from any toxic effects by catalyzing the decompostion of H2O2 without the production of Oxygen free radicals. Catalase takes two molecules of hydrogen peroxide and converts them to two water molecules plus a molecule of oxygen gas. Hydrogen peroxide is a toxic molecule. Amount of solution in test tube is called the controlled variable. Controlled variables must be carefully monitored and kept equal in your experiments - otherwise they could mess up your experiment by making your results false or unreliable.
Catalase is considered an endoenzyme because it is produced and used within a cells metabolic machinery. Since it breaks down superoxide molecules it must work inside the cell.
Corrosive substance can be removed with contrasting pH substances. Because corrosive substances are acidic or low on the pH scale, the solution to remove them needs to be high in the pH scale, or basic. Ammonia is an example of a basic solution.
A statement that presents a possible solution to a problem is the hypothesis. You construct a hypothesis, then work to prove it. Basic geometry concentrates on proving various nypotheses.
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