A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
The negative stain does not colorize the cells in the smear because the bacterial surface reples the negatively charged chromogen thus, the cell remains unstained while the background is colored
Yes.
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
A cervical smear is a screening test used to detect pre-cancerous and cancerous cells by taking a sample of cells from the cervix.
the smear decolourised more time than it required
Yes, a smear that is too thick can cause overlapping of cells and make it difficult to differentiate individual cells, while a smear that is too thin may not have enough cells to adequately examine and may result in a false negative interpretation of the sample. It is important to aim for a smear that is of optimal thickness for accurate interpretation.
By applying to many cell in a smear it would become difficult to study the morphology of single cell as they will form clumps for e.g. if we want to study some cocci then it would be difficult to estimate there occurence i.e. either they are in chain or in cluster or may be diplococci or monococci.
epithelial cells
Polymorphonuclear cells are white blood cells. These are a nonspecific finding on a pap smear result. Contact your health care provider to find out if any further evaluation is required in your specific situation.
Epithelial cells are a normal part of the anatomy. They are the type of cell checked in a pap smear. If they are present, it's a sign that the pap smear got a decent sample.
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.
Gram variability refers to a characteristic of certain bacteria that can exhibit variability in their response to Gram staining, appearing as a mix of both Gram-positive and Gram-negative characteristics. This variability can make the identification of these bacteria challenging because their staining characteristics may not be consistent.
A cervical smear is a screening test used to detect pre-cancerous and cancerous cells by taking a sample of cells from the cervix.
the smear decolourised more time than it required
A pap smear is classified in a number of ways. The adequacy of the smear is evaluated in terms of the types and numbers of cells collected. The cervical cells are evaluated for normalcy. Lastly, if any additional findings are noted, such as yeast, these are reported as well.
Endocervical and/or squamous cells are normal on a pap smear. This notation on your result means the test was complete.
The point of the gram stain is to differentiate between two things, with out both positive and negative gram cells there would be nothing to differentiate between, defeating the purpose of the process.
ASC-US. ASC-H ,AGC ,AIS,LSIL ,AND HSIL cells Detect