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Q: Why doesn't ninhydrin react with polypeptides?
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Why egg albumin and casein did not react ninhydrin reagent?

It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.


Is ninhydrin the same as super glue?

NO, ninhydrin is not the same as super glue


Why proline amino acid gives yellow colour in reaction with ninhydrin?

Yes. Within "The Chemical Reactions of Amino Acids / Reactions of Amino Groups" there are several examples - one of which is the ninhydrin reaction. A very widely applied reaction of the alpha amino group [that is the N in the peptide bond -CCN-CCN-], it is used to estimate the quantity of amino acids [in a sample] in very small amounts. All amino acids and polypeptides with a free alpha group react with ninhydrin and yield [or produce] an intensely purple colored product - except for Proline and Hydroxyproline {both in which the alpha amino group is termed to be 'substituted' - something to do with carbon rings} which "yield derivatives with a characteristic yellow color." See also 'Schiff's bases'.


Does ninhydrin has flaws?

Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.


What is the principle of heat coagulation test for proteins?

The principle of the heat coagulation test for proteins involves the reaction of amino acids and Ninhydrin. When these two react together under high temperatures, fingerprints can be seen.

Related questions

Why egg albumin and casein did not react ninhydrin reagent?

It should react to this reagent. However it must be HEATED before it will react.This is due to the fact that when Ninhydrin is heated it stabilizes and the reacts with the -NH2 groups on the amino acid.


What questions have been raised about ninhydrin?

Ninhydrin is a chemical used to expose latent fingerprints, but its use is declining because of the slow reaction time it takes for it to react with the oil in the fingerprints. You can use heat to speed the process up but there are other faster options.


Is ninhydrin the same as super glue?

NO, ninhydrin is not the same as super glue


Why proline amino acid gives yellow colour in reaction with ninhydrin?

Yes. Within "The Chemical Reactions of Amino Acids / Reactions of Amino Groups" there are several examples - one of which is the ninhydrin reaction. A very widely applied reaction of the alpha amino group [that is the N in the peptide bond -CCN-CCN-], it is used to estimate the quantity of amino acids [in a sample] in very small amounts. All amino acids and polypeptides with a free alpha group react with ninhydrin and yield [or produce] an intensely purple colored product - except for Proline and Hydroxyproline {both in which the alpha amino group is termed to be 'substituted' - something to do with carbon rings} which "yield derivatives with a characteristic yellow color." See also 'Schiff's bases'.


How does copper react in water?

It doesnt


Does ninhydrin has flaws?

Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.


How does soda react to heat?

no it doesnt blow up


What are Polypeptides are assembled from?

Polypeptides are chains of amino acids.


What does silicon react to?

Silicon doesnt really react a lot unless it is under extremely high temperatures


What is the molecular formula of ninhydrin?

Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4


Are polypeptides carbohydrates?

No, Polypeptides are chains of amino acids and Proteins


What substances are found on the human skin that react with ninhydrin resulting to stain or violet coloration on the skin?

Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a substance intended to discover the existence of Ammonia or anything that contains Amines. When this substance reacts with these Amines a blue-purple stain will be observed. Since, the skin contains amino acid, a blue-purple stain will be noticed.