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its because
E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose
ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.
in nutrient agar:- 2-3mm, grey colonies,moist circular,convex colonies are seen.in blood:-grey colonies.in macConkey agar:-pale /yellowcolonies are seen.in deoxycholatecitrate agar:-colourless,smooth,shiny colonies salmonella are seen.
How do colonies on the surface of a pour plate differ from those suspended in the agar?
How do colonies on the surface of a pour plate differ from those suspended in the agar?
MacConkey's agar is a differential media used to differentiate between lactose fermenting and lactose non-fermenting bacteria. E.coli is a lactose fermenter whereas Pseudomonas is a lactose non-fermenter.MacConkey's agar contains lactose as fermentable sugar and when it is fermented the pH of the medium decreases which is registered by neutral red (a pH indicator).Lactose fermenters such as E.coli produce pink colonies whereas lactose non-fermenters such as Pseudomonas produces colorless colonies. So the colors of E.coli and Pseudomonas colonies are different on MacConkey's agar.
Probably yes, E. coli grows much faster than most microbes
white colonies
it is used to count the colonies
Because of their ability to grow on specific environment & the amount of oxygen needed...(e.g. anaerobes, aerotolerants will grow in agar).
Well, first of all, the colonies growing on the surface of the agar medium are aerobic . . . that is, they need air - Oxygen - to survive. The anaerobic colonies growing within the agar medium may simply be slower growing or maturing, for some reason. Perhaps that is simply due to their being anaerobic.