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To sterilise effectively the water in the autoclave needs to reach a temperature of 120°C. Since water at atmospheric pressure boils at 100°C, a higher pressure is needed to reach that temperature.

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Q: Why high pressure needed in autoclave?
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Can you autoclave 1M HCl solution?

No. Vapor pressure is too high. Used a closed microwave vessel system with temperature and pressure control. A 1500 psi vessel is needed.


What is 15 pounds pressure in autoclave?

Steam autoclave's work at 30 to 35 PSI, the previous answer of 15 PSI is for a pressure cooker. A pressure cooker cannot be used as an autoclave, the temperature is not high enough to cause sterilization. Anything less than 121C / 250F (30 PSI) is incapable of killing microorganisms. Also the pressure vesal of a pressure cooker cannot handle the added pressure needed to sterilize, IE: adding weight to the release valve will just cause the cooker to explode (so don't try it).


What is an autoclave reactor?

a reactor is generally termed as an autoclave reactor,when it is a high pressure batch reactor.mostly this kind of reactors are used for sterilization the reactants..


What is the main principal of autoclave?

steriling the acessories by introducing saturated steam with required pressure high temperature under the influence if high pressure that is 121 degree celsius temp and 15 ATM pressure.


What is the device use to sterilize equipment and supplies by subjecting them to high pressure saturated steam?

An Autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam.


Why are cans in a retort cooled under pressure?

A retort pouch (laminated plastic-metal) is cooked at 240-250 degrees F under high pressure in an autoclave. it is not cooled under pressure.


The advantages and disadvantages of autoclave sterilisation?

An autoclave uses high-pressure steam to sterilize items placed in it.The advantage is it kills germs very well.The disadvantages are they're expensive, they take a while to run a cycle through, and not every beauty tool can be run through an autoclave.


How do you test the efficiency of an autoclave in microbiology?

An Autoclave is a piece of equipment used for sterilization purposes and requires quarterly calibration. There are three types of calibrations to be done - timer, temperature and pressure. To calibrate the timer, post an appropriate correction factor directly on the autoclave. Post a correction factor on the autoclave control panel if the temperatures were different, and for pressure post a correction factor on the autoclave if it is not true.


What is a sterilizing machine called that steams and heats under pressure?

Autoclave


What is the Principle of autoclave in biotechnology?

An autoclave is piece of equipment that is usually used to sterilize objects and supplies by subjecting them to high pressure saturated steam at 121 °C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. The sterilization occurs by three mechanisms: temperature, pressure, and thermal oxidation. The high temperature will kill most microorganisms because they cannot tolerate the temperature - essentially it "cooks" them. Viruses, which may not be considered "organisms" are also broken down by the temperature and proteins can be denatured. The pressure will cause penetration of membrane walls of organisms, disrupting or breaking the walls, forcing the steam into them, and screwing up the osmotic pressure. Some biological materials will also experience thermal oxidation - they react with residual air present in the autoclave or with other oxidizers present in the autoclave. As the reactions occur, the biological materials are broken down or destroyed.


What shape is an autoclave?

A vessel in which high heat and pressure [generated by converting water to steam] is used to sterilize objects.A simple, basic descripton is that it is a fancy PRESSURE COOKER!TThe development of autoclave by Chambarland in 1884 trememdously stimulated the growth of microbiology. Water is boiled to produce steam, which is released through the jacket and into the autoclave's chamber the desiered temperature and pressure are usually 121Co 15 punds of pressure.It DependsSince the inside of an autoclave is a pressure vessel, it is generally cylindrical until the size makes casting one piece impractiable, and building from parts begins; some autoclaves are the size and shape of walk in closets! The outside of an autoclave is usually a square or rectangle around the pressure vessel and contains piping, insulation and control valving. I have seen autoclaves that were shaped like a slice of bologna, with the same approximate height to radius ratio.


What is a pressure cooker-like apparatus used to sterilize instruments called?

Autoclave.