The most important part of the plant tissue culture is to maintain the sterile environment and the plant growth in axenic condition, as the seed growth on a very slow rate, any contaminant will cause the seed to unable to growth. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon completely overwhelm the small plant piece that we are trying to clone.
As axenic cultures are derived from very few organisms, or even a single individual, they are useful because the organisms present within them share a relatively narrow gene pool. In the case of an asexual species derived from a single individual, the resulting culture should consist of identical organisms (though processes such as mutation and horizontal gene transfer may introduce a degree of variability). Consequently, they will generally respond in a more uniform and reproducible fashion, simplifying the interpretation of experiments
The need of pure culture of bacteria to characterize an individual species. Pure culture are also important to study the morphology and physiology of individual bacterial species, their biochemical behaviour and response to different compounds like antibiotics, which all can me alter by the influence of other species if prestent (in mixed culture) and also for isolating and studying of their molicular structure i.e. DNA or RNA. Some common ways to obtain a pure culture of bacteria are: 1 The spread plate technique. 2 The pour plate. 3 Streak plate technique.
if contamination is noted in a subculture sample, what should be to obtain a pure culture
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Streak plating and picking a single colony for the culturing in a broth.
As axenic cultures are derived from very few organisms, or even a single individual, they are useful because the organisms present within them share a relatively narrow gene pool. In the case of an asexual species derived from a single individual, the resulting culture should consist of identical organisms (though processes such as mutation and horizontal gene transfer may introduce a degree of variability). Consequently, they will generally respond in a more uniform and reproducible fashion, simplifying the interpretation of experiments
i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture
People can obtain knowledge of another culture's religion and magic by reading books, attending cultural events, participating in rituals or ceremonies, and engaging in respectful conversations with individuals from that culture. It is important to approach learning with an open mind and to always show respect for the beliefs and practices of others.
control the contamination
Culture media should be specific to avoid contamination of unwanted microorganisms and to obtain a pure culture of microorganisms.
The need of pure culture of bacteria to characterize an individual species. Pure culture are also important to study the morphology and physiology of individual bacterial species, their biochemical behaviour and response to different compounds like antibiotics, which all can me alter by the influence of other species if prestent (in mixed culture) and also for isolating and studying of their molicular structure i.e. DNA or RNA. Some common ways to obtain a pure culture of bacteria are: 1 The spread plate technique. 2 The pour plate. 3 Streak plate technique.
if contamination is noted in a subculture sample, what should be to obtain a pure culture
=) hi
W important for you to obtain professional credentials in your chsen field?
Streak plating and picking a single colony for the culturing in a broth.
They obtain a sense of self through ethics and culture. This ethics and culture are passed on from generation to generation and has become part of them.
in order to obtain the perfection of wisdom