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Mg2+ complexes with the single stranded DNA that is to be amplified, and becomes the substrate of DNA polymerase. In other words, it helps in the binding of primer (and the subsequent target DNA) to the template DNA. Different volume of Mg2+ exert different complex-forming capabilities, and thus affects the end product of PCR.
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∙ 14y ago
MgCl2 is used in PCR because it acts as a cofactor for the DNA polymerase enzyme, thereby enabling the enzyme to function optimally. The Mg2+ ions help stabilize the DNA strands and facilitate the binding of primers to the template DNA during the amplification process. The concentration of MgCl2 can affect the efficiency and specificity of the PCR reaction.
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What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
PCR stands for?
PCR stands for Polymerase Chain Reaction.
Does mgcl2 is an aqous solution?
yes mgcl2 is aqous solution
Is SiCl plus Mg---MgCl2 plus Si Balanced?
Mg + MgCl2
What is molarity of soultion containing 12.5g of Mgcl2 in 200 ml soultion?
MgCl2 Mg = 24.30 2Cl = 70.906 FW = 95.21 (Remember SigFigs) 12.5g ÷ 95g of MgCl2 = 0.131 moles of MgCl2 200mL = 0.200 L 0.131 moles of MgCl2 ÷ 0.200 L = 0.656 Molar Concentration
What is the function of mgcl2 in pcr?
it enhance the reaction
Role of magnesium chloride in PCR?
Magnesium chloride is a crucial component in the polymerase chain reaction (PCR) as it is required for the activity of the DNA polymerase enzyme. Magnesium ions help stabilize the DNA template-primer complex and are essential for the enzymatic activity of the DNA polymerase, allowing for successful DNA amplification during PCR. The optimal concentration of magnesium chloride can vary depending on the specific DNA polymerase being used and the PCR conditions.
Role of additives in PCR?
Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What is the common name for mgcl2?
MgCl2 is magnesium chloride.
105 g of MgCl2 contains how much mol MgCl2?
MgCl2=95grams per mole so 105g/95g is 1.10 mols of MgCl2
What is the molarity of 80g magnesium chloride dissolved in 250ml of water?
MgCl2 Molarity = moles of solute/Liters of solution ( 250 ml = 0.250 L ) Get moles MgCl2 80 grams MgCl2 (1 mole MgCl2/95.21 grams) = 0.8402 moles MgCl2 Molarity = 0.8402 moles MgCl2/0.250 Liters = 3.4 M MgCl2 ----------------
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the correct way to write the formula for magnesium chloride?
The synthesis equation for MgCl2 is Mg + Cl2 -> MgCl2. The decomposition reaction is MgCl2 - heat-> Mg + Cl2.
Draw an atomic representation for MgCl2 on octet rule?
MgCl2
