because STR only requires small pieces of DNA (2-5 base pairs long). it is fast and automated wheres RFLP can take up to a month to accomplish. STR is also better because it allows the use of the Polymerase Chain Reaction (PCR). whereas RFLP requires large amounts of non-degraded DNA and automation is not possible.
it can be as little as a single cell if using low copy number (LCN) analysis however if you are looking for results that are a little more reliable its ussially over 1 nano gram, that's using STR (short tandom repeate) analysis. if using something like RFLP (ristriction fragment length polymorphism) it would be alot more then that, however that technique is hardly used anymore. visually repisented the smallest amount of DNA to produce an STR profile is the size of a full stop . i hope this helps thegreatmizuti
sometimes, there isn't enough DNA found at a crime scene to be properly analyzed by forensic scientists. RFLPs are used to make exact copies of the DNA sequence so that it can be better analyzed in the lab.
RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
A short tandem repeat or STR is a type of polymorphism, where short sequences of DNA are repeated. It is a useful tool in forensics because the number of times a DNA sequence is repeated for a given STR varies between individuals.
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
Do you mean "RFLP" if so its, restriction fragment length polymorphism. (DNA analysis)
A gene is a specific sequence of DNA that contains the instructions for making a particular protein or RNA molecule, while RFLP (restriction fragment length polymorphism) is a technique used to detect variations in DNA sequences by cutting DNA with restriction enzymes and separating the resulting fragments by size. In other words, a gene is a functional unit of DNA, while RFLP is a method to study genetic variation at the DNA level.
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
it can be as little as a single cell if using low copy number (LCN) analysis however if you are looking for results that are a little more reliable its ussially over 1 nano gram, that's using STR (short tandom repeate) analysis. if using something like RFLP (ristriction fragment length polymorphism) it would be alot more then that, however that technique is hardly used anymore. visually repisented the smallest amount of DNA to produce an STR profile is the size of a full stop . i hope this helps thegreatmizuti
What can be the main limiting factor in the use of RFLP?
RLFP is an acronym for Resriction Fragment Length Polymorphism. RLFP analysis is used to identify changes in a genetic sequence that occurs at a site where a restriction enzyme cuts. RFLP's can be used to identify specific mutations and also trace inheritance patterns!
A method known as RFLP (restriction fragment length polymorphism) analysis can be used to make a DNA fingerprint.
Stephen James Gray has written: 'The genotyping of neisseria meningitidis by restriction fragment lengthpolymorphism (RFLP) analysis'
it can be as little as a single cell if using low copy number (LCN) analysis however if you are looking for results that are a little more reliable its ussially over 1 nano gram, that's using STR (short tandom repeate) analysis. if using something like RFLP (ristriction fragment length polymorphism) it would be alot more then that, however that technique is hardly used anymore. visually repisented the smallest amount of DNA to produce an STR profile is the size of a full stop . i hope this helps thegreatmizuti
It depends on your game and your class.
#include<stdio.h> int main() { char str[100]; int i; printf("Please enter a string: "); // gets(str); // fgets is a better option over gets to read multiword string . fgets(str, 100, stdin); // Following can be added for extra precaution for '\n' character // if(str[length(str)-1] == '\n') str[strlen(str)-1]=NULL; for(i=0;str[i]!=NULL;i++) { if(str[i]>='A'&&str[i]<='Z') str[i]+=32; else if(str[i]>='a'&&str[i]<='z') str[i]-=32; } printf("String in toggle case is: %s",str); return 0; }
#include<stdio.h> #include<conio.h> #include<string.h> int main() { int tmp,i; char str[30]; printf("Enter any string: "); gets(str); for(i=0; str[i]!='\0'; i++) { if(str[i-1]==' ' i==0) { if(str[i]>='a' && str[i]<='z') str[i]=str[i]-32; else if(str[i]>='A' && str[i]<='Z') str[i]=str[i]+32; } printf("%c",str[i]); } getch(); return 0;}