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Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
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When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What in gluconeogenesis during the postabsorptive state amino acids and what are converted to glucose?
glycerol
What types of buffer are used for hplc?
Buffer solutions reduces the ionization during the elution in the column and gives a long life for Reverse phased columns.
What is the role of glycerol in the lysis buffer?
To protect protein during thawing and freezing
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the function of TEB buffer in gel electrophoresis?
Restriction buffer maintains the pH in a range suitable for enzyme activity, as well as supplying salt cofactors required for catalysis. Since different restriction enzymes require varying salt conditions and pH, a single compromise buffer can be used that strikes a balance between conditions preferred by the various restriction enzymes. (Spec. compromise restriction buffer)
Which fragements travel the fastest and farthest during electrophoresis?
The smallest and lightest fragments.
Are glycerol suppositories safe during pregnancy?
Yea it sure is
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
During digestion fat is changed into?
Fatty acid molecules and glycerol
Is it safe to take glycerol suppositories during pregnancy?
no like your mama
What are the problems faced during the preparation of 3 percent agarose gel?
The principle one I can think of is you can't. I can't imagine that amount of agar melting, let alone staying liquid or without bubbles long enough to pour. The standard electrophoresis gel is 0.8 grams/100 mls ya it is correct.bubbles wil remain for long time.even during taking out of comb breakage of well wil be thr but that can be noted only after loading the sample.bcoz well breakage is thr means samples wil come out of the well .or it just spreads in the buffer leads to the loss of pcr product
What molecules are released when fat undergoes a hydrolysis reaction?
A fat molecule forms when glycerol joins with three fatty acids as three water molecule are removed during dehydration reaction.
What in gluconeogenesis during the postabsorptive state amino acids and what are converted to glucose?
glycerol
