There is a variety of reasons you would want to isolate D.N.A these include:
1. Medicine
- to test newborn babies for genetic disease by sequencing the relevant gene e.g. cystic fibrosis
- studying the gene involved in causing cancer to identify the mutation causing disease
2. Crime
- analyze forensic evidence to establish events and identify people present at a crime scene and the positive identification of a corpse
3. Food Safety
- trace the origin of an outbreak of food poisoning
4. Biotechnology
- isolate genes of interest and insert them into plants to give them: better test / pesticide resistance / increased nutrient value, or into bacteria and use them as 'factories' to produce high value products like the peptide insulin.
Hope that helped in some way!
There is a variety of reasons you would want to isolate D.N.A these include:
1. Medicine
- to test newborn babies for genetic disease by sequencing the relevant gene e.g. cystic fibrosis
- studying the gene involved in causing cancer to identify the mutation causing disease
2. Crime
- analyze forensic evidence to establish events and identify people present at a crime scene and the positive identification of a corpse
3. Food Safety
- trace the origin of an outbreak of food poisoning
4. Biotechnology
- isolate genes of interest and insert them into plants to give them: better test / pesticide resistance / increased nutrient value, or into bacteria and use them as 'factories' to produce high value products like the peptide insulin.
Hope that helped in some way!
One must try their level best in minimizing contamination while isolating DNA. If the materials used (like reagents and tubes) are contaminated with bacteria for example, the final DNA sample will also contain a certain amount of bacterial DNA contamination. This negatively affects any further experiments. Therefore, it is strictly advisable to use sterile lab-ware while isolating DNA.
1. cloning
2. identification of a person
3. use it to get your self-tested for various diseases
4. identification of paternity and maternity cases in forensic level
o Genetic testing
o Body Identification
o Analysis of forensic evidence
Cause different bacteria has different DNA composition. Isolated colony assures that the bacterial DNA you are extracting is coming from one specie only.
for further transcription
replication forks separate and replicate DNA
DNA Polymerase
Yep!
This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.
- Filtration is a method of separation when we want to separate solids from a liquid.- Distillation is a method of separation when we want to separate the components of a liquid mixture, relying on the differences between boiling points.
replication forks separate and replicate DNA
because we would
DNA Polymerase
Salt is used to separate DNA.
No, DNA is held in the nucleolus a separate part of the cell.
If heated to a hundred degrees, chromosomal DNA would denature. Meaning the it would come apart and the complementary DNA strands would separate. One way to get DNA to spool (around a glass rod for example) is to remove it from the cell and precipitate it in solution. This can be done with with help of sodium chloride and isoamyl alcohol.
Yes
telomeres
Sodium chloride help to precipitate and separate DNA.
you would want to look into mitosis. This process will explain more about the copying of DNA.
I do not know tell me
for self pleasure (if you catch my drift)