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Why pcr products are precipitated before sequencing?
The PCR product are precipitated before sequencing to increase the concentration of tamplet DNA.
Can weak acids be precipitated from solutions of their salts by lowering the pH?
Yes this may be possible.
Is it possible to isolate DNA from bone?
Yes it is possible to isolate DNA from bone.
Why do you need to break down the membrane during DNA extraction?
The DNA is present inside the cell and the cell is encapsulated by the plasma membrane. In, eukaryotes, the DNA is further localized in the nucleus and that is also surrounded my nuclear membrane. If one would not rupture the membranes, the DNA will not come in the solution and could not be precipitated.
What is the role of proteins in allowing DNA to fit in a cell?
The protein histone, and attendant proteins, spool the DNA tightly around their complexes and then further wind these complexes into tighter and tighter shapes. About two meters worth of DNA per cell is held in these histone complexes.
Why pcr products are precipitated before sequencing?
The PCR product are precipitated before sequencing to increase the concentration of tamplet DNA.
What is the purpose of using detergent in extracting plant DNA?
Detergent denatures (breaks up) proteins, which can then be precipitated and the DNA can be isolated
Why 70 percent ethanol is used for DNA pellet washing?
In the process of DNA isolation, ethanol is used to preciputate the DNA or bring the DNA out of solution. Once precipitated, DNA appears as a white cottony mass that can be seen with the naked eye. This precipitated DNA is then spun down and re suspended in the appropriate buffer suitable for storage.
The proteins in the chromosomes provide spool-like support on which DNA is wound These structures are called?
nucleosomes
A portion of a DNA molecules wound around a spool of histone proyein is called?
cell cycle
Would chromosomal DNA spool if it was heated to 100 degrees?
If heated to a hundred degrees, chromosomal DNA would denature. Meaning the it would come apart and the complementary DNA strands would separate. One way to get DNA to spool (around a glass rod for example) is to remove it from the cell and precipitate it in solution. This can be done with with help of sodium chloride and isoamyl alcohol.
A portion of a DNA molecule wound around a spool of histone protein is called a?
Such a structure is called a Nucleosome
If you were able to spool the DNA you could see that it is stringy and has the consistency of thick syrup or mucus Based on what you know about the molecule why do you think it has this consistency?
dna has a comparatively long structure
What is the purpose of alcohol when extracting DNA?
According to me, we use alcohol because DNA is insoluble in alcohol, it aggregates together, giving a pellet in centrifugal and we can see a precipitated DNA with naked eyes (that we suppose to see in experiment i.e DNA extraction)....
Can weak bases be precipitated from solutions of their salts by increasing the pH?
Yes this may be possible.
Can weak acids be precipitated from solutions of their salts by lowering the pH?
Yes this may be possible.
How many meter welding possible in 15 kg 1.2 mm wire spool?
1.2mm mig weighs 0.0087kg/meter therefore 1.724km per 15kg spool
