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Why is oil added to view a bacterial stain under 100x resolution in microscope?
Wiki User
∙ 12y ago
Bacteria are very tiny. Using a high powered microscope lens in necessary to view them properly, but at such high powers and close focusing, the 'picture' is often skewed by the air itself and it is easy to damage the lens. The oil provides a clearer focus by eliminating the air pocket between the lens and the specimen and also helps protect the lens from scratching and cracking.
Wiki User
∙ 9y ago
Wiki User
∙ 12y agoOil is added to view a bacterial stain under 100x resolution so as to help eliminate the air between the cover glass and the objective lens , thus helping to reduce or minimize the distortion.
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What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
What color is coccus under a microscope?
All bacteria, including cocci, are colorless under the microscope unless you use a light filter or a stain. If you use a light filter or a stain then the color depends on which wavelength the filter is for or which type of stain you are using.
Why are basic stains attracted to the bacteria itself?
The bacterial cell wall has a negative charge. The basic stain has a positive charge. Since they have opposite charges, the bacterial cell wall and the basic stain are attracted to each other; hence the basic stain dyes the bacteria.
Why is it important to add iodine to cheek cells?
There is no complex chemical reason why iodine is added to a cell. It is simply added to stain a cell to make it easier to study under a microscope, as it gives some contrast to the cell to make certain organelles stand out that would be unclear or invisible without this stain. There are, however, many other stains that can be used, for example Haematoxyline and Eosin (H&E) staining which is used often to identify and study cancerous cells.
Why are stains such as iodine used to observe cells under the microscope?
Iodine is used to stain the cell. It makes each component of the cell more visible, especially the nucleus.
Is flagella viewable through an electron microscop or a light microscope?
Eukaryotic flagella are visible through a light microscope. Bacterial flagella are only visible with a light microscope if they are specially stained with a mordant and a flagella stain.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
What is the function of a stain in microscope work?
As the cells are transparent, the components cannot be seen clearly through a microscope. We stain the cell so that we can see the components of the cell clearly through a microscope.
What color is coccus under a microscope?
All bacteria, including cocci, are colorless under the microscope unless you use a light filter or a stain. If you use a light filter or a stain then the color depends on which wavelength the filter is for or which type of stain you are using.
What is it called to add dye to a specimen under a microscope?
to stain.
What is a stain that you can use to see things in a microscope easier?
iodine
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
How can you see the parts of a cell?
You can see parts of cells by microscopy.A light microscope will reveal detail with a resolution down to about 0.2 micrometers. More detail becomes visible using various techniques, such as staining or phase contrast microscopy.An electron microscope improves the resolution to about 2 nanometers. What is seen in an electron micrograph is the shadow left by heavy metal atoms in the stain, which has attached to cell structures.
How can you see part of a cell?
You can see parts of cells by microscopy.A light microscope will reveal detail with a resolution down to about 0.2 micrometers. More detail becomes visible using various techniques, such as staining or phase contrast microscopy.An electron microscope improves the resolution to about 2 nanometers. What is seen in an electron micrograph is the shadow left by heavy metal atoms in the stain, which has attached to cell structures.
What can you see a bacteria with?
im assuming you mean idividual cells, not a bacterial colny, as that's often visable with the naked eye. depending on what you want to see, you can stain the bacteria with specific dyes and then veiw them under a light microscope. for example, DAPI is often used if you wish to visualise the DNA of the cells.
Why are basic stains attracted to the bacteria itself?
The bacterial cell wall has a negative charge. The basic stain has a positive charge. Since they have opposite charges, the bacterial cell wall and the basic stain are attracted to each other; hence the basic stain dyes the bacteria.
How are sputum cultures performed?
.the sputum must be collected into a sterile container. Once in the laboratory, each culture type is handled differently. Bacterial culture.sputum is smeared on a microscope slide for a Gram stain. for mold or yeast, a fungal culture is done.
