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Why is pure culture necessary for enterotube tests but not for DNA probes?
For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.
What is the role of radioactive phosphorus in DNA technologies?
To make DNA probes
What are Molecules of DNA are referred to as?
Its not seekers it could possiably be probes.
What is genomic chips?
Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip
How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
Why is pure culture necessary for enterotube tests but not for DNA probes?
For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.
In what field are TaqMan probes used?
TaqMan probes are used in the field of molecular biology. They are used in many medical labs around the world for purposes of gene expression and DNA research.
What is the role of radioactive phosphorus in DNA technologies?
To make DNA probes
Rna and DNA probes bind specifically to the single stranded DNA of their target?
true
What are Molecules of DNA are referred to as?
Its not seekers it could possiably be probes.
What is genomic chips?
Genome chips are miniaturized plates containing hundreds of microscopic wells on their surface. These wells contain DNA probes. DNA probes are basically stretches of cDNA from a particular genome. When genomic DNA isolated from an organism is allowed to interact with the cDNA probes, come probes bind to the genomic DNA while others do not (depending on complementarity. A laser light is used to read each well and look at what sequences are bound. This information is valuable to scientists who can determine changes in gene expression based on the information obtained from a gene chip
How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
What is difference between DNA probe and primer?
http://answers.yahoo.com/question/index?qid=20080514100528AAqFDXF
Scientist test for alleles that cause genetic disorders by?
using DNA probes
What are molecules that seek out and identify a particular molecule or piece of DNA referred to as?
Probes
Molecules that seek out and indentify a particular molecule or piece of DNA are referred to as?
probes
If the DNA in a DNA extraction is not pure what other types of molecules might be present?
If the DNA is not pure, contaminants include RNA and proteins
