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Ringers solution contains a salt content similar to what is found in the cells of a bacteria. This prevents the bacteria under going osmotic stress and subsequent bursting of the cell walls.
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What is a ten fold serial dilution?
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
Can you use plain normal saline solution for the dilution of the drug ampicillin?
yes
What test is used to further identify unknown gram negative?
Serial dilution technique
Is Saline NaCl?
Not exactly. Saline solution is sterilized and is a dilution of sodium chloride (NaCl) - meaning that the NaCl is dissolved in deionized water. The solution is 9 grams of sodium chloride (NaCl) dissolved in 1 liter of water.
What are the advantages and disadvantages of the serial dilution agar plate procedure?
Both force you to read your lab manual.
Why is saline used in serial dilution?
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
Why is saline used in bacterial serial dilution?
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
What is a ten fold serial dilution?
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
What is the differences between a streak plate technique and a serial dilution technique?
what is serial dilution and spread plate technique
Can you use plain normal saline solution for the dilution of the drug ampicillin?
yes
What test is used to further identify unknown gram negative?
Serial dilution technique
Is Saline NaCl?
Not exactly. Saline solution is sterilized and is a dilution of sodium chloride (NaCl) - meaning that the NaCl is dissolved in deionized water. The solution is 9 grams of sodium chloride (NaCl) dissolved in 1 liter of water.
Who invented serial dilution method?
Robert koch
Can sterile saline be used interchangeably with injectable saline?
no sterile saline cannot be used because strile saline is different from injectable saline. strile saline is used for irrigating the wound and injectable saline in given intravenously
What is the purpose of serial?
A common design for estimating the concentrations of compounds in biological samples is the serial dilution assay, in which measurements are taken at several different dilutions of a sample, giving several opportunities for an accurate measurement. Curren tly, serial dilution is a standard tool in the fields of toxicology and immunology.Serial dilution helps to choose a dilution which is relevant to our experiment.Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. But equally the equipment has a detection limit so we can't dilute it to much, or if it is too diluted the experiment might not work.
What are the advantages and disadvantages of the serial dilution agar plate procedure?
Both force you to read your lab manual.
Is dilution an acceptable way to dispose of the used copper chloride solution?
Is dilution an acceptable way to dispose of the used copper chloride solution
