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Why is staining bacterial components useful in strain identification?
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How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification. In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
Can the API20E kit used for the identification of any bacterial strain?
Currently, the API20E kit is just used to study gram negative bacteria, especially the Enterobacteriaceae.
How will you separate all the components of a mixture of sand and salt in water-?
add all the components and mix them in a glass. strain the water from strainer in other glass and the sand will be separated. boil the water until it evaporates fully. water will be separated and the salt will be left.
What important question remained unanswered by griffith experiment?
Assuming you mean the Frederick Griffith bacterial experiment, the question left unanswered was how the rough strain (less harmful) bacteria transformed into the smooth strain (lethal) bacteria. When he injected live rough strain bacteria, the mouse lived. When he injected live smooth strain bacteria, the mouse died. But if dead smooth strain bacteria was injected, the mouse lived. So if either live rough strain or dead smooth strain could be injected without killing the mouse, then it would stand to reason that one could inject both, the mouse should live. But the mouse died. So he figured the rough strain was somehow taking on the characteristics of the smooth strain bacteria, perhaps by being in close proximity to dead smooth strain bacteria, but he didn't know why. We now know that the smooth strain DNA was somehow getting grafted into the rough strain bacteria and making it able to create a coating which prevented the immune system (of the mouse in this case) from killing it. In case you mean the John Howard Griffin racial change experiment, the question left unanswered was how to stop racism. While his experiment was not completely successful, he gained a number of insights into the experiences of men of color in America. He was able to speak to people in both groups, but yet, he was not able at that point to get them to reconcile with each other.
What is transformed from rough strain bacteria to smooth strain cells?
DNA
How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification. In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
Can the API20E kit used for the identification of any bacterial strain?
Currently, the API20E kit is just used to study gram negative bacteria, especially the Enterobacteriaceae.
What Bacterial infection is caused by an unusual strain of E. coli?
Dysentery
What did griffith find out when he accomplishd his experiment on streptococcus pneumoniae?
The amount of thymine equals the amount of adenine in DNA.
What is shear strain?
Shear strain is the components of a strain at a point that produce changes in shape of a body (distortion) without a volumetric change. That is, the tangent of the angular change in orientation of two initially perpendicular lines . Approximately equal in magnitude to the angle itself in radians for infinitesimal strains.
What was Frederick griffin contributions to genetics?
Frederick Griffith, a British bacteriologist, focused on the epidemiology and pathology of bacterial pneumonia. He showed that Streptococcus pneumonia, implicated in many cases of lobar pneumonia,[2] could transform from one strain into a different strain. This was later identified as DNA.
What research has been done on anemarrhena?
Laboratory studies have shown that anemarrhena can effectively eradicate infections caused by Staphylococcus aureus, the bacterial strain that often causes lung infections.
What cell structure found in plant and bacterial cells but not animal cells called?
In Bacterial cells (prokaryotes) the genetic material is not enclosed in a nucleus, instead, it is a single strain of DNA in a circle with no end. Bacterial cells may also contain a few small strands of DNA, known as plasmids. Bacterial cells have a cell wall as well as a membrane. They also have cytoplasm as all cells do
How will you separate all the components of a mixture of sand and salt in water-?
add all the components and mix them in a glass. strain the water from strainer in other glass and the sand will be separated. boil the water until it evaporates fully. water will be separated and the salt will be left.
Define shear strain in civil engineering?
Strain shows how much longer a beam becomes after applying a force in a chosen direction.Strain = change of length of the the beam / original length of the beamIn case of Shear Strain force is applied only parallel to the surface of the beam (not normal to it).The same principal can be applied not only to beams, but to other civil engineering components as well.
What happens if you drive with your 4 wheel drive on in good weather conditions?
It wears out tires, puts excessive strain on the 4wd components and uses a lot more fuel.
The s strain and the r strain of S pneumoniae are different in that?
The S strain produces a capsule but the R strain does not
