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∙ 14y agoIt is because of the thickness of the peptidoglycan cell wall of the bacteria.
Gram positive bacteria have a larger/thicker cell wall.
It is this cell wall which retains the stain of the crystal violet(primary stain) and carbol fuschin(counterstain).
Gram negative have a very thin cell wall. So when the Acetone solution is applied in between stains the crystal violet is washed out of the gram negative bacteria. As it is only left on for a few seconds the gram positive bacteria can still retain the crystal violet solution due to the cell wall thickness.
The counterstain is then added. because the gram negative bacteria have been 'unstained' by the acetone the fuschin (pink stain) is absorbed and therefore shown when looked at under a microscope.
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∙ 14y agoThe difference in color between gram-positive and gram-negative bacteria is due to the structure of their cell walls. Gram-positive bacteria have a thick layer of peptidoglycan that retains the crystal violet stain, giving them a purple color. In contrast, gram-negative bacteria have a thin layer of peptidoglycan and an outer membrane that does not retain the stain, causing them to appear pink or red after counterstaining with safranin.
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∙ 14y agoGram positive bacteria appear blueish and Gram negative bacteria appear redish, after staining with Gram stain.
The reason why these bacteria react differently to gram staining procedure is because of differences in the structure of the cell wall between gram positive and negative organisms. Alcohol is used to counterstain the cell and gram positives retain the stain, whereas gram negative don't.
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∙ 13y agoThe cell wall of a bacterium is made up of short chain amino acids and sugars linked together called peptidoglycans. Depending on their thickness they will take up the grams stain. Gram+ve bacteria have a layer of peptidoglycans up to 5x thicker than Gram-ve bacteria. This layer retains the Crystal Violet/Iodine stain in Gram+ve bacteria whereas in Gram-ve it does no but they will take up the methyl red counterstain. Also Gram-ve bacteria have extra layers of lipo-polysaccharides and these also retain the counterstain.
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∙ 10y agoGram-positive bacteria have a thick mesh-like cell wall which is made up of peptidoglycan (50-90% of cell wall), which stains purple. Peptidoglycan is mainly a polysaccharide composed of two subunits called N-acetyl glucosamine and N-acetyl muramic acid. As adjacent layers of peptidoglycan are formed, they are cross linked by short chains of peptides by means of a transpeptidase enzyme, resulting in the shape and rigidity of the cell wall. The thick peptidoglycan layer of Gram-positive organisms allows these organisms to retain the crystal violet-iodine complex and stains the cells as purple
Gram-negative bacteria have a thinner layer of peptidoglycan (10% of the cell wall) and lose the crystal violet-iodine complex during decolorization with the alcohol rinse, but retain the counter stain Safranin, thus appearing reddish or pink. They also have an additional outer membrane which contains lipids, which is separated from the cell wall by means of periplasmic space.
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∙ 12y agoB'coz gm positive bacteria get stained with the colour of safranine...
The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane
Positive gram staining indicates that the bacteria retain the crystal violet dye and appear purple under the microscope, while negative gram staining indicates that the bacteria do not retain the dye and appear pink or red. This difference is due to variations in the bacterial cell wall structure, with positive gram bacteria having a thick peptidoglycan layer and negative gram bacteria having a thinner peptidoglycan layer.
Gram positive bacteria cell walls have a thick layer of Peptidoglycan and no periplasmic space. Gram negative bacteria cell walls have inner and outter cytoplasmic membranes with a periplasmic space in between. these also have a thin layer of Peptidoglycan. The outter cytoplasmic membrane of gram negative bacteria contains lipopolysaccharides.
Gram-positive and Gram-negative refer to two different types of bacteria distinguished by the structure of their cell walls. Gram-positive bacteria have a thick layer of peptidoglycan in their cell walls, while Gram-negative bacteria have a thinner layer of peptidoglycan surrounded by an outer membrane. This difference affects how the bacteria react to the Gram staining technique used in microbiology.
Candida albicans is a yeast and belongs to the fungal kingdom, so it does not have a gram classification like bacteria. Gram staining is a technique used to differentiate between gram-positive and gram-negative bacteria based on the composition of their cell walls.
They have different cell walls.
The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane
The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane
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one is positive, the other is negative
positive ions carry positive charge and negative ions carry negative charge
Positive looks like this + Negative looks like this -
Positive correlation has a positive slope and negative correlation has a negative slope.
Each of them is the negative of the other one.
positive yhe porlar
The difference between any numbers is always positive.
gram- negative and gram- positive bacteria differ in their response to different antibiotics