0
To Stop The Reaction
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Why do you use trichloroacetic acid in glycogen extraction?
Trichloroacetic acid is widely used in biochemistry for the precipitation of macromolecules.
'why we use glycerol in estimation of boric acid in given sample'?
boric acid does not react with NaOH directly. When glycerol is added, the mixture behaves as monobasic and so it can be titrated with alkali using phenolphthalein
What is the country that Purafect Protease is used in?
England. UK
To test wether a sample contains proteins what reagent should be used?
The Bradford reagent (Coomassie) is commonly used to detect if a sample contains protein. Coomassie will react with aromatic amino acids (phenylalanine, tyrosine and tryptophan) to turn from a dull red color to a bright blue color. This assay is dependant on the amount of aromatic amino acids present, but works well as a "quick and dirty" indicator of the presence of a protein. The bicinchonic acid assay (BCA assay), while more expensive than the Bradford assay, more accurately detects the presence of the peptide bond present in proteins, so it can be used to not only detect proteins which lack aromatic amino acid residues, but also can be used to more accurately determine the concentration of protein in a sample as not all proteins have the same amount of aromatic amino acids.
Why do you use trichloroacetic acid in glycogen extraction?
Trichloroacetic acid is widely used in biochemistry for the precipitation of macromolecules.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
What are the uses of trichloroacetic acid?
Trichloroacetic acid is widely used in biochemistry for the precipitation of macromolecules such as proteins, DNA and RNA. Its sodium salt is used as a weedkiller. Solutions containing trichloroacetic acid as an ingredient are used for cosmetic treatments such as chemical peels and tattoo removal and the treatment of warts, including genital warts. It can kill normal cells as well. It is considered safe for use for this purpose during pregnancy[3].Salts of trichloroacetic acid are called trichloroacetates. Reduction of trichloroacetic acid results in dichloroacetic acid, a pharmacologically active compound that shows promise for the treatment of cancer.Source: Wikipedia
Is manitol used in the assay of boric acid?
yes
What amino acid residues are detected when the spectrophotometric assay is used to quantify proteins?
In Lowry spectrophotometric assay, tryosine, tryptophan, cysteine, histidine, and asparagine.
What is washing powder used in?
Protease enzymes
'why we use glycerol in estimation of boric acid in given sample'?
boric acid does not react with NaOH directly. When glycerol is added, the mixture behaves as monobasic and so it can be titrated with alkali using phenolphthalein
What is an assay ton?
An assay ton is a standard quantity used in assaying, the act of ascertaining the proportion of a particular metal in an ore or alloy, typically equal to 29.16 grams (short assay ton) or 32.6 grams (long assay ton).
Why acetic acid is used in copper sulphate assay by iodometry method?
For the best results reaction should take place in the slightly acidic solution (pH around 4-5), correct pH is obtained by addition of acetic acid.
What enzyme is used in washing powder?
Protease enzymes
What is the country that Purafect Protease is used in?
England. UK
Name a use for both protease and lipase?
Protease and Lipase are both enzymes that are secreted during digestion. Protease enzymes break down proteins into amino acids, which can then be used by the body for growth and repair. Lipase enzymes break down lipids (fats and oils) into glycerol and fatty acid chains; which form cell membranes and can be respired to release a lot of energy (double the amount per mole of carbohydrate.
