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What is extracted from cells for DNA microarray technology?
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
What is the meaning of CDNA microarray?
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
What is DNA microarray data analysis used for?
DNA microarray analysis is a technique used to match up two strands of DNA. It is used in paternity tests and in criminal investigations in which a perpetrator's DNA was found at the crime scene.
What is the purpose of DNA microarray analysis?
Otherwise known as "microarray chips," DNA microarray are used to determined the genetic makeup of a given tissue sample. By shining various bands of light on these chips, the gene(s) in the tissue are expressed in the form of a particular color.
What are the steps of DNA microarray assays?
Step 1: Isolate the mRNA. Step 2: Make cDNA by reverse transcription using fluorescent labeled nucleotides. Step 3: Apply the cDNA mixture to a microarray with a different gene in each spot. Step 4: Rinse off excess DNA; scan for fluorescence.
What enzyme do you need to get a copy of DNA from mRNA?
Reverse transcriptase is the enzyme used to synthesize DNA from mRNA.
When is mRNA used?
when DNA is sending a message
What is a DNA microarray?
In a DNA microarray, thousands of DNA fragments are spotted onto a glass slide. Usually, it is a fragment of each gene (or cDNA) that is spotted onto these glass slides. Each of these fragments will be used for a hybridization reaction with mRNA that is collected from tissue samples. To determine the expression of these tissues, mRNA is collected and turned into cDNA. Fluorescent labeled nucleotides are added to the sample so that they will be detectable under a colored laser. Two samples are usually compared to each other. The labeled cDNAs are allowed to hybridize with the genomic fragments that were spotted onto the glass slides. The cDNAs from each sample will be able to simultaneously hybridize with the target sequences.
DNA is used as a blueprint to make this molecule?
In the process of transcription, DNA is used as a blueprint to make m-RNA which codes for a specific protein.
What is used to carry the genetic code from DNA to the ribosomes?
The mRNA carries the genetic code needed to make a protein to the ribosome from DNA via microtubules.
What does reverse transcriptase use from DNA?
Reverse transcriptase use mRNA to form DNA. mRNA
What are the pros and cons of integrating DNA microarray or chip technology into the forensic DNA laboratory?
no profit to answer
