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Why must warm agar be cooled down at 45 47 celsius before agar plates are poured?
Wiki User
∙ 7y ago
Why we cool agar before pouring depends on WHY we are pouring it. If we are pouring a gel or standard media for streak culture then we allow some cooling to take place because we don't want to burn our fingers. If we are pouring a pour plate we allow a lot of cooling to take place so we don't kill our microbes through heat steralization. As my teacher used to say "boiled bugs don't grow".
Wiki User
∙ 14y ago
Wiki User
∙ 13y agoIf you're adding antibiotics (especially ampicillin) you have to let the agar cool so that it doesn't destroy the antibiotics. If you're adding bacteria to the agar to make pour plates then you have to let it cool down so that the bacteria don't die.
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Why is culture medium cooled 48 temperature before is poured?
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
Why doesn't a penny melt in water?
A penny is a solid material that is made out of copper and the copper is melted and poured into a mold and cooled.
Why is nutrient jelly heated before being poured into petri dish?
to kill the micro - organisms
When 2 cups of hot chocolate one at 55 degrees Celsius and the other at 60 degrees Celsius the other at 60 degrees Celsius are poured in a bowl why will the temperature of the mixture be between?
Assuming no loss of heat to the bowl or the air, the temperature of a mixture of two amounts of a substance will be the weighted average of the temperatures of the components - weighted according to their quantities. So the resulting temperature of the above mixture wil be between 55 and 60 degrees C.
If gasoline poured into a tank an chemical change?
Gasoline being poured into a tank is not a chemical change.
Why would the temperature of hot chocolate be between 50 degree celsius and 60 degree celsius if poured into a bowl?
Because it was between 50 and 60 degrees before pouring it into the bowl.
What is pour plating?
Pour plating is a method of separating one species of bacteria from another by diluting one loopful of organism into three liquefied nutrient agar plates, with the hopes that one of the plates poured will provide an ideal sample for isolation.
Will it damage my van if i accidentally poured windshield washer fluid into the radiator reservoir?
No it won't hurt it. If you only poured a little in the radiator I wouldn't give it another thought. If you poured a quart or more in there you probably need to get your radiator flushed before the temperature starts hitting any extremes. Aluminum engine parts can be a little touchy if they aren't heated and cooled uniformly.
Marty poured hot water into his cup and let it cool off what were the particles of matter doing as the water cooled?
hot bioilling water
What is a good sentence using the world poured?
She poured a very expensive perfume all over her body before the party.He poured more milk into his cup.
How do you make crayons?
Crayons are made of paraffin wax and pigment. The wax is hot when the pigment is added and mixed. Then the mixture is poured into crayon molds and cooled.
Why is culture medium cooled 48 temperature before is poured?
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
Marty poured hot water into his cup and then let it cool off. what was happening to the paticiles of matter in the cup as the water cooled off?
the water molecules are getting cold
What is the process to make synthetic plastic?
To make a synthetic plastic, plastic pellets are melted and then poured into a mold. The mold forms the product, and then the product is cooled and the mold opened.
Why must the temperature of the agar be no lower than 45C and no higher than 50C when being poured into the plates?
the bacteria will dentaure and die off
What should be poured across the deck before each voyage?
Wine.
Why do obisidian rocks have no crystals?
because the magma cooled so fast the little pieces of cooled magma bonded already so there is not enough space for the little pieces to grow into crystals. Similar to concrete the bricks are the pieces of already cooled magma. But then the concrete is immediately poured onto the brick t=and cooled in 1 second and hardened. That does not allow space for the bricks to grow as everything is bonded and no more magma is left to add to the pieces to make them bigger.
