It is supposed to help you center on the specimen.
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Ocular Lens (eyepiece) or Stage
You use the low power lens to find the specimen with a microscope. Once you have found the specimen on low power, you can switch to high power for greater magnification. Just be sure to use only the fine adjustment knobs for focusing on high power so the high power objective won't hit the slide.
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When viewing a specimen, you must always use the lower power objective lenses first. Low power lens gives the widest field of view and makes it easier to find the specimen when you look through the microscope. Finding the specimen at high power, without first centering it in the field of view at low power, is nearly impossible.
start by focusing with the lowest lens to make sure the slide is right and you can see the specimen them proceed to the next lens power and focus again just to make the view clear then proceed to the highest and focus one more time
It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO.
First put the specimen into focus using a low power lens, then switch to a higher power lens if needed.
1. Use the eyedropper to place a drop of water on the center of a microscope slide2. Use the tweezers to position the letter "e" in the center of the drop.3. Holding the cover slip at a 45 degree angle, lower it slowly over the drop of water. Here's what the letter "e" looks like under low and high power. As you can see, the specimen appears to be upside down and backwards. As you can see, when you switch to high power the field of view DECREASES. Field of view is the amount of the specimen you are able to see. The higher the power, the less you see of the entire specimen. This is why it is always better to begin focusing on the lowest power possible. Beginning on the lowest power gives you the Greatest field of view, which means you will be able to see the entire specimen. For example, you most likely learned the alphabet in preschool, yet it is difficult to tell that the specimen under high power in the picture above is the letter "e".
First put the specimen into focus using a low power lens, then switch to a higher power lens if needed.