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Young cultures must be used when doing a gram stain to get more accurate results. The cell was is the part of the bacterial cell that is most involved with gram staining because it holds the crystal violet.

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Guido Larkin

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2y ago

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Why must fresh bacterial cultures be used in a Gram stain?

Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate


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