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What else can I help you with?
Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
What gram reaction would you expect if you used a culture that was growing for 72 hours?
If a culture has been growing for 72 hours, it is likely that most of the bacterial cells would have undergone multiple rounds of cell division and may have varying degrees of cell wall synthesis. As a result, it would be challenging to predict a specific gram reaction without additional information or performing a Gram stain test on the culture.
What is a gram variable bacteria?
A gram variable bacteria is a type of bacterium that does not consistently stain as either gram-positive or gram-negative. This variability can make it challenging to identify using traditional Gram staining techniques.
It is normal to have gram positive cocci in urine culture?
Finding gram positive cocci in a urine culture could indicate a possible urinary tract infection (UTI), especially if present in significant quantities. The specific type of gram positive cocci identified can help determine the cause and guide treatment. However, it is important to correlate these findings with the patient's symptoms and clinical context for an accurate diagnosis.
How will a gram stain sample appear if the culture is contaminated?
If a gram stain sample is contaminated, it may show a mixed population of bacteria with varying gram reactions, such as both gram-positive and gram-negative organisms. This can lead to unclear or confusing results, making it difficult to identify the target bacteria. Contamination can also introduce artifacts or unexpected cell types, further complicating interpretation. Overall, contamination undermines the reliability of the gram stain results.
Why must young cultures be used when doing staining?
Young cultures must be used when doing a gram stain to get more accurate results. The cell was is the part of the bacterial cell that is most involved with gram staining because it holds the crystal violet.
Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
Suppose you are viewing a Gram stained field of red rods and purple cocci through the microscope What do you conclude?
The rods will be gram negative, the cocci are gram positive: most likely, the slide was made from a mixed culture of bacteria, or the culture was contaminated.
Why must fresh bacterial cultures be used in a Gram stain?
Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate
What is S Acin in a urine culture report?
S Acin on a urine culture report likely refers to Staphylococcus saprophyticus, which is a type of bacteria commonly found in the gastrointestinal tract and can sometimes cause urinary tract infections in young women. It is important to consult with a healthcare provider for proper diagnosis and treatment if S. saprophyticus is identified in a urine culture.
Sputum Culture-gram positive cocci?
Sounds pretty typical.
What gram reaction would you expect if you used a culture that was growing for 72 hours?
If a culture has been growing for 72 hours, it is likely that most of the bacterial cells would have undergone multiple rounds of cell division and may have varying degrees of cell wall synthesis. As a result, it would be challenging to predict a specific gram reaction without additional information or performing a Gram stain test on the culture.
Can you do a gram stain from 3 day old nutrient agar plate or should i do anohter t streak and let it grow for one day nd then do gram stain?
there should be no problem with doing a gram stain on a 3 days old bacteria strain unless your working with spore making bacteria, then you would need to do another streak, unless your familiar with spore stain methods but you should keep the bacteria cold at 4 degrees if you dont want them to die.
What is a gram variable bacteria?
A gram variable bacteria is a type of bacterium that does not consistently stain as either gram-positive or gram-negative. This variability can make it challenging to identify using traditional Gram staining techniques.
Is B subtilis a gram positive or gram negative bacterium?
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
How is the Gram stain technique be of value in establishing purity of a culture of bacteria?
deffirent bacteria show different response to gram stainig procedure so bacteria are classified into two groups:a(gram positive bacteria)b(gram negitive bacteria)
