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Q: Why ninhydrin test is positive for all proteins?
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Why ninhydrin shows positive test for amino acid and protein?

Ninhydrin reacts with ammonia, a primary amine, or a secondary amine (amino acids have a primary or alpha amino group, except for proline which has a secondary amino group). They all turn purple/blue right away upon heating with ninhydrin.Remember that a protein has a amino terminal and a -COOH terminal. The ninhydrin will react with the amino terminal giving a very light blue or violet color (more often than not you will see no color change). Upon further heating you may notice an increase in the intensity of the blue/violet. this is due to the heat denaturing the protein, thereby exposing more -NH2 groups for the ninhydrin reagent to react with


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Is it possible to get 3 false positives in a roll... I'm about 7 days late I took 3 hpt and all 3 were positive... so it possible that all 3 tests were false positives.. help?

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What is the positive inside rule?

The positive inside rule is a general rule for membrane proteins. Because of the mechanism involved in transmembrane domain insertion (regions of the protein which cross the membrane), membrane proteins tend to have more residues which can take on a positive charge on the inside of their cells. So, almost all, if not all membrane proteins have more solvent exposed Lysine and Arginine residues on the sides of the transmembrane domains which rest inside of the cell in comparison to the number which rest on the outside of the cell.