I think pBR322 has a replication module from E coli plasmid colE1 ,which permits plasmid replication even when chromosome replication and cell division are inhibited by amino acid starvation and chloramphenicol, as a result, under such condition each cell accumulates several thousands copies of the plasmids up to 3000, so that one litre of bacterial culture easily yields a milligram of plasmid DNA.
HIgh copy number means that the bacteria will replicate many copies of the plasmid. That means that you suppose to get a high yield of plasmid at the end.
high
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers.
Those which do not nurture their offspring and those which spawn in an aquatic environment
The turnover number for catalase is extremely high. One molecule of catalase can easily convert 400 million molecules of hydrogen peroxide to H2O.
HIgh copy number means that the bacteria will replicate many copies of the plasmid. That means that you suppose to get a high yield of plasmid at the end.
Many Pseudomonas plasmids are transmissible to E. coli, but the copy number is plasmid-dependent. Some plasmids are of low-copy number whereas some are high-copy number, and we should also consider copy-number-incompatibility. It could not be said that plasmids which can replicate in E.coli and Pseudomonas are of high copy number.
The copy number reflects the average number of copies of a certain plasmid inside a host cell. The higher the copy number, the more efficient the plasmid is at replicating itself. Researchers using plasmids as vectors usually choose high copy number plasmids as their vectors since you can get a large number of plasmids from relatively fewer cells in less time.
control of copy number in plasmids
high
Copy of diploma from Thrall High School Thrall,tx
While in the Army I completed my high school and was given a Washington State High School diploma. How can I get a copy of it?
you get free copy of high school diploma online information at www.online-high-school-diploma.com.
In order to get a copy of your high school diploma from 1983, you will need to visit with the high school in which you graduated from. They are the only people who would have such documents.
no
A plasmid is a tall, placid being that lives in the deepest crevis of ben nevis. A plasmid hardly ever speaks English, and instead communicates with fellow plasmids in a high, mincing, undecipherable shout that goes something like this: GETOFFYOURLAZYBACKSIDEANDDOSOMERESEARCHAMNDSTOPUSINGWIKIANSWERS. This is harmful to human ears, and, in case of contact with plasmids, one should pull one's bowler hat right down overy one's ears and proceed to run away with swift swiftness.
I am ignacio cuevas from philippines ,my original high school diploma was lost ,were can i get anoter copy and how to get?