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What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use.
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
What is col plasmid?
A plasmid (free bit of DNA) in bacteria that produces colicin to kill other strains of bacteria.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
A bacterial cell that has taken up plasmid DNA is?
A bacterial cell that has taken up plasmid DNA is transformed.
What is last step in the production of a recombinant DNA plasmid?
The last step in the production of a recombinant DNA plasmid is joining the DNA. This is done by adding DNA ligase to joint DNA fragments.
A single piece of coiled DNA is known as?
A coiled piece of DNA is called a plasmid, found in prokaryotic cells.
What is a synonym for plasmid?
A plasmid is essentially extra-chromosomal self-replicating DNA
Human DNA is spliced into the?
human DNA are spliced into plasmid
What is the function of plasmid DNA?
Plasmids have small pockets of DNA in them.
What is recombinant Plasmid?
A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. ... Researchers can insert DNA fragments or genes into a plasmid vector, creating a so-called recombinant plasmid. This plasmid can be introduced into a bacterium by way of the process called transformation.
How is plasmid DNA inserted into the cell where the DNA originally come from?
by transformation
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
