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The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification.
However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
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Do plasmid act as a carrier of foreign DNA and is needed to clone a gene?
Yes, plasmids can act as carriers of foreign DNA and are commonly used in gene cloning. Plasmids are small, circular DNA molecules that can replicate independently from the host cell's genome. They can be manipulated to include a foreign gene of interest, and when introduced into a host cell, the plasmid can replicate along with the host cell's DNA, allowing for the amplification and expression of the cloned gene.
DNA is used to form bonds between the inserted gene and the plasmid DNA?
ligase
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use.
What is difference between DNA probe and primer?
http://answers.yahoo.com/question/index?qid=20080514100528AAqFDXF
When plasmids are used to transfer foreign DNA to other cells the plasmid is called?
When plasmids, are used to transfer foreign DNA to other cells the plasmid is called a
Do plasmid act as a carrier of foreign DNA and is needed to clone a gene?
Yes, plasmids can act as carriers of foreign DNA and are commonly used in gene cloning. Plasmids are small, circular DNA molecules that can replicate independently from the host cell's genome. They can be manipulated to include a foreign gene of interest, and when introduced into a host cell, the plasmid can replicate along with the host cell's DNA, allowing for the amplification and expression of the cloned gene.
DNA is used to form bonds between the inserted gene and the plasmid DNA?
ligase
What structures does a bacteria form that is used to transfer DNA?
Pilus, plasmid
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
What are plasmid genes?
A plasmid is an extra chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently from the chromosomal DNA http://en.wikipedia.org/wiki/Plasmid I think this is far use.
A plasmid is a type of?
Plasmid is extrachromosomal DNA capable of self replication.
How can you increase amplification?
are you referring to DNA amplification using PCR
A bacterial cell that has taken up plasmid DNA is?
A bacterial cell that has taken up plasmid DNA is transformed.
How is plasmid DNA similar to the DNA found in chromosomes?
The plasmid DNA is a small self replicating particles other than the DNA found in the chromosomes. The both DNA are self replicating but the plasmid DNA is very important in techniques like nucleic acid hybridizations , radio graph micro assay, PCR and many other. Both of the DNA are used in recombinant technology.
What is last step in the production of a recombinant DNA plasmid?
The last step in the production of a recombinant DNA plasmid is joining the DNA. This is done by adding DNA ligase to joint DNA fragments.
