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Can relative retention time changed?
No
What is the effect of flow rate on retention time in HPLC?
When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
Why do substances travel different distances in chromatography?
Due to the fact that different substances possess different retention time (they do differ from their size,shalpe,mol wt etc.)
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What is the difference between relative retention time and retention time?
the difference between retention time and retention factor is that the retention time is a quatitative value where`s the retention factor is a qualitative on.
Can relative retention time changed?
No
What is RRT and RRF in hplc?
In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor
What is the effect of flow rate on retention time in HPLC?
When you increase the flowrate of the carrier gas, the retention times decrease. Just like when you increase the temperature of the column. Both of these conditions are sometimes necessary for substances that would otherwise have very long retention times.
Why do substances travel different distances in chromatography?
Due to the fact that different substances possess different retention time (they do differ from their size,shalpe,mol wt etc.)
How to calculate Relative retention time?
Divide the retention time of the peak of ineterest (ex. 14.8 min.) by the retention time of the main peak (ex. 15.9 min.) 14.8/15.9 = 0.93 Therefore your RRT is 0.93 Remember, any peak with an RRT <1 elutes before the main peak, and any peak with an RRT >1 elutes after the main peak! What is RRT & RRF in hplc
What will be the effect of pH on retention time?
Retention time decrease with an increase in pH.
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What is the Retention factor of caffeine?
it depends on the retention time, void time and reagents that you were used on your chromatograph.
What does retention time mean?
The retention time represents the time it takes to an analyte to pass from the column inlet to the detector.
Which great lake has the shortest retention time?
Lake Erie has the shortest retention time of about 2.7 years
Are there any advantages in increasing retention time?
The retention time would increase becasue longer distance would be travelled by the analyte!
