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Why steam heating is done in acid fast staining and not heat fixing?
Wiki User
∙ 14y ago
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
Wiki User
∙ 14y ago
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Preparation of pyruvic acid from tartaric acid?
on heating the tartaric acid, the tartaric acid forms a keto acid which is known as pyruvic acid C4H6O6 ------KHSO4/heating------> C3H4O3 (tartaric acid) (Pyruvic acid)
Where can you get nitric acid?
schools in flupe motgomery have a heating system called pop123 heating it has nitric acid
What is the practical importance in a medical lab of the acid-fast stain?
By this technique, we can diffentiate the acid fast and non acid- fast bacteria. The non acid-fast bacteria are M.tuberculosis and N.asteriodes. They are the causative agents for tuberculosis and nocardiosis respectively. The acid fast staining or the Ziel- Nielsen's staining is the only procedure to find out the above mentioned pathogens.
What happens to boric acid when you burn it?
On heating, Orthoboric Acid above 370K forms metaboric acid, which on further heating yields Boric Acid.
Equation of burning of gas evolve in the heating of oxalic acid in the presence of sulfuric acid?
Oxalic acid undergoes ready decarboxylation on mild heating to give carbon monoxide,carbon dioxide and water(vapour).
What is staining in a pool?
Acid
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
Preparation of pyruvic acid from tartaric acid?
on heating the tartaric acid, the tartaric acid forms a keto acid which is known as pyruvic acid C4H6O6 ------KHSO4/heating------> C3H4O3 (tartaric acid) (Pyruvic acid)
Where can you get nitric acid?
schools in flupe motgomery have a heating system called pop123 heating it has nitric acid
How do you decolourise schiffs reagent?
With periodic acid during the steps of staining slides.
What is the practical importance in a medical lab of the acid-fast stain?
By this technique, we can diffentiate the acid fast and non acid- fast bacteria. The non acid-fast bacteria are M.tuberculosis and N.asteriodes. They are the causative agents for tuberculosis and nocardiosis respectively. The acid fast staining or the Ziel- Nielsen's staining is the only procedure to find out the above mentioned pathogens.
Other methods of fixing nitrogen in the soil?
Acid rain.
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
How do you get rid of the muratis acid you put into your pool to get rid of staining?
Acid reduces Ph So by adding potash you will neutralise the acid and regain the correct Ph
What is an acid-fast pathogen?
An acid-fast pathogen is a bacteria that is harmful to humans. They have cell walls that contain mycolic acid which is a lipid. Common Gram type staining techniques wont work with these cells. A special stain carbolfuchsin is used to penetrate the wall.After staining you wash with acid alcohol if the stain remains it is acid fast if it washes out it is non-acid fast.Mycobacterium tuberculosis is a well known acid-fast pathogen
What is a primary stain?
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
