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Why taq polymerase is strored at very low temperature as it is thermostable?
Wiki User
∙ 14y ago
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
Wiki User
∙ 14y ago
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How often does DNA polymerase match bases incorrectly?
very infrequently. when it does a mutation occrs
A source of heat-stable DNA polymerase is?
A DNA polymerase is one of the crucial enzymes when DNA is synthesised. It is also the only enzyme needed when making DNA in the test tube, using a molecular biology technique known as PCR.In this reaction, the other enzymes that nature uses are are replaced by cycles of heating and cooling, up to 95 degrees Celsius. The DNA polymerase consists of protein, so normal DNA polymerase is of course destroyed in the heating step. The first PCR's were performed by opening the tube in each step and adding a tiny amount of fresh enzyme. Using heat-stable DNA polymerases made the technique a lot more practical. The enzymes used were taken från a type of archae (not exactly a bacterium, but almost) that live in hot springs and whose proteins all are very stable even in extreme temperatures. The archaeon is called "Thermus aquaticus", hence the name of the common lab DNA polymerases "Taq polymerase":To sum it up. A heat-stable DNA polymerase is a kind of DNA polymerase found in archaea living in hot springs, and of much use in the molecular biology lab.
Are microorganisms live in tempe?
micro-organisms live at a suitable temperature,they die on a very high and low temperature.....
Can polymerase catch and correct every reptication error?
Yes. Initially, DNA replication makes 1 mistake in a 100,000. Like spell check, DNA polymerase comes in and removes errors in base pairs and correct them by adding the right ones. After DNA polymerase checks the new strand for errors, the end result is 1 mistake in a billion. If this didn't occur, mutations would surely take place in out body.
How are the accuracy of DNA and RNA assured?
Most DNA Polymerases and RNA polymerases have what is known as "proof-reading activity". This is the ability of the enzymes to check what bases they have added during DNA replication (in the case of DNA Polymerase) or transcription (in the case of RNA Polymerase), and if an error is found, splice it out and replace it with the correct base. The mode of action depends on the enzyme in question - some use endonucleases, and some use exonucleases; some work 5'-3' while others work 3'-5'. Also note that I said MOST polymerases have proof-reading capabilities...there are a few which do not (or don't proof-read very well).
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Role of Taq polymerase in PCR?
taq polymerase is special because it is very stable at high temperatures and will not denature even at the 90 degree step of pcr. taq polymerase is so heat stable because it was extracted from the bacterium thermus aquaticus, which is found in hot springs and geezers
Application of Polymerase Chain Reaction to Adenovirus and Herpavirus and Pox-virus?
it is very good
How often does DNA polymerase match bases incorrectly?
very infrequently. when it does a mutation occrs
What is the explanation for the Role of RNA polymerase?
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polymerase, RNA polymerase codes for the complementary nucleotides to a DNA strand. Instead of thymine though, uracil codes with adenine. This coded mRNA strand then travels from the nucleus to the ribsome where translation occurs - the result is protein made from an amino acid chain. To answer your main question - RNA polyermase adds the complementary nucleotides to the DNA strand using uracil instead of thymine. hope that helps :)
How does Polymerase Chain Reaction work?
Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.
A gas that burns at a very high temperature?
which gas burns at a very high temperature
What is the temperature in the summer for Chad?
The temperature is very hot
What is the temperature range in Ontario?
The average temperature is
What is the Neptune's surface temperature?
The surface temperature on Neptune is very very cold. it is - 391 F.
Polymerase chain reaction was developed so that scientists could?
Polymerase chain reaction (PCR) is used to make many, many copies of DNA. For example, if there is a very small drop of blood at a crime scene, a PCR machine can replicate the DNA over and over and over again so that there is enough of it to make a comparison to a person. Before PCR, if there wasn't a lot of blood, they were out of luck. It was a very brilliant idea. Answer The polymerase chain reaction amplifies small amounts of DNA. Large amounts of DNA are required to perform further analysis such as DNA fingerprinting.
What is the meaning of amplification of gene?
Gene amplification is the process of taking a very tiny sample (in some cases as few as one molecule of DNA) and rapidly generating a sample of millions or billions of identical molecules of DNA. This process must be entirely acellular, so that the sample is not contaminated with unrelated DNA. The most commonly used technique of gene amplification makes use of PCR (polymerase chain reaction) that makes use of a DNA polymerase enzyme derived from a virus. PCR only requires adding this enzyme and nucleotides to the DNA then cycling the temperature of the mixture up and down a little, each of these temperature cycles doubles the number of copies of the desired DNA molecule.
