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sticky ends are more specific and easier to ligate
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What are the steps of using recombinant DNA technology?
1 Isolate DNA 2 Cut DNA with a restriction enzyme 3 Mix the DNA's and join then together by using DNA ligase 4 Insert the recombinant plasmid into a host bacterium 5 Allow the bacterium to reproduce
What end of a growing strand are DNA nucleotides added to?
5' end (nucleotides are added from 3' toward 5')
If you were to stretch all the DNA in all our cells end to end from the earth to the sun how many rounds can we go?
we can go about 5 times from the earth to to sun
What type of mechanical wave is produced by pushing sharply on the end of a spring toy?
Buoyancy
Why are GMOs produced?
Basically, a lab person takes the DNA of one organism, cuts it with restriction enzymes at specific sites, which will make the DNA ladder have hanging single strands at the end. The same enzyme cuts the DNA of the organism you want to stick in the original dna. then, these strands go together.
What can restricted enzymes leave?
Restriction enzymes can leave blunt or sticky ends. This will depend on where they cut the DNA, if they cut at the same point on both strands, they will leave a blunt end, if they cut at different points, they will leave sticky ends. For example: (| represents point of cut) Blunt end ATC|GCTA TAG|CGAT Sticky end A|TCGCTA TAGCGA|T
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
What are the types of cloning?
In general, sticky end cloning and blunt end cloning
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
What are stick ends?
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
If the sticky end of a nucleotide sequence is TTAA what is next?
If the sticky end of a sequence is TTAA, it can bind to a DNA molecule with the sequence AATT
A dna fragment with a sticky end that reads -attcg will bind with another dna fragment with a sticky end that reads?
ATTCG signify adenine (A), thymine (T), cytosine (C) and guanine (G). The bonding pairs are AT, GC when DNA replicates. Therefore the fragment ATTCG will bind to TAAGC.
List the two nucleotide sequence that are complementary to the sticky end sequence on the human DNA?
It is GGCC and CCGG!
What does blunt means in angle?
blunt means dull or rounded, for example: "A knife has a sharp end and a blunt end ."
Use E co RI and MseI in AFLP analysis?
Chop DNA into pieces using you restriction enzyme(s) of choice. Add adapter to sticky end, you know the sequence of the sticky end as it corrisponds to the restriction enzyme used. Use a primer for the adaptor and amplify the DNA with PCR. Ta dah you just amplified somthing you didn't have a primer for. Run the amplified DNA on a gel and you can see changes between your samples.
What does the term sticky end refer to in genetic engineering?
I tried to show this with actual letters showing the overlap of double strands, but this stupid site does not allow proper spacing and " corrects " capitalization, so I will have to tell you verbally. When a plasmid or gene section is digested a overhang is created in the double strand that exactly matches the under hang of the other piece of strand.
At the end of DNA replication two new strands of DNA have been produced giving a total of?
Two new DNA chains are formed at the end of DNA replication, both identical in sequence to the template (or parent) chain. These chains are composed of 2 complimentary strands. It is important to note that of the newly formed DNA chains, one strand is the same as the template strand and the other one is a newly synthesized one.
