because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain
other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
to fixed the blood smear
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
It dries the smear and fixes the cells to the slide
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
methanol for fixing the smear and acetone free to avoid cell membrane lysis
to fixed the blood smear
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
It dries the smear and fixes the cells to the slide
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
The smear will not adhere well if there is grease on the slide.
smear will be washed( no smear will be left on the slide)
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony