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Why is agar added to nutrient media?
a thickener.
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
How ampicillin synthesised?
how ampicillin and amoxycillin synthesised
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
The plasmid pARA-R has been genetically modified for what purpose?
ampicillin resistant
How do you make pitri dish gel agar?
Agar is generally made by mixing the powdered form with varying ingredients. The powder is based on seaweed extract for nutrient agar, and nutrient agar is generally a base for most other agars - eg. Horse Blood Agar is nutrient agar with horses blood added; Choc agar has defibrinated (cooked) horses blood added.
Why is agar added to nutrient media?
a thickener.
What two compounds added to amoxicillin and ampicillin to strenghten them against microbe resistance?
nesosporin and bacitracin
Why is ampicillin added to the pound medium?
Ampicillin is an antibiotic that is usually used as a reporter gene in cloning. A plasmid containing the ampicillin resistance gene (as well as another target gene within the plasmid) is introduced into the bacterial host. If the bacterium has taken up the plasmid and is expressing the plasmid, it will be resistant to ampicillin. LB is used as a growth medium and ampicillin to verify the plasmid is within the bactrium. No growth means no plasmid in the bacterial host...
What can be added to suppress bacterial growth on a fungi based Media?
Salts can be used to anybody but Ariel got on water but only fungi based Media such as potato dextrose agar PDA, some antibiotics are effective and can be used to suppress the growth of bacteria E.g penicillin, Ampicillin, streptomycin, Amoxicillin & rifampicin.
Why is ampicillin important?
ampicillin is a antibiotic
How ampicillin synthesised?
how ampicillin and amoxycillin synthesised
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
The plasmid pARA-R has been genetically modified for what purpose?
ampicillin resistant
Why glycerol is added in cetrimide agar?
as a source of carbon which important for bacterial growth
How do ampicillin resistant E. coli combat the lethal effects of the ampicillin antibiotic?
The pGlo plasmid contains an ampicillin resistance gene. This gene encodes an enzyme, β lactimase, which enzymatically degrades ampicillin. Therefore, bacteria that take up the plasmid (transformants) become resistant to ampicillin.
What is the concentration of bacteriological agar normally used in making solid media?
Agar is added in a concentration of 1.5% to solidify liquid media. Although there are different concentrations of agar used to create different conditions such as in Sabouraud's Agar and semisolid agar.
