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what is the role of HCL in the DNase experiment
HCL to acidify the mixture
a seperating funnel is used to seperate mixtures
because hcl contains cl2
1.0 M = x mol HCl /.018 L of solution x = .018 mol HCl Since HCl --> H+ and Cl- in a 1 to 1 to 1 ratio, then you will also have .018 mol of H+ in solution.
what is the role of HCL in the DNase experiment
take the root tip (about 1 cm )and put it in dil. HCL solution for ten min. take the tip out and put in acetocarmine stain for 1min. take out the root put it in water bto remove extra stain .
HCL to acidify the mixture
a seperating funnel is used to seperate mixtures
because hcl contains cl2
An example; preparation of sodium chloride in laboratory: NaOH + HCl = NaCl + H2O
1.0 M = x mol HCl /.018 L of solution x = .018 mol HCl Since HCl --> H+ and Cl- in a 1 to 1 to 1 ratio, then you will also have .018 mol of H+ in solution.
HCl (Hydrochloric Acid) is highly acidic (it is stomach acid!). Therefore, HCl will actually damage and potentially destroy the proteins, so it is not a great idea to denature proteins using something that will destroy them
get some Zn metal (or use a new penny) and get some HCl. You will see the H2 gas bubble and the Zn will corrode
HCl is used to destroy the substances that unite the cells so that the cells spread easily and you can see one layer of cells on your slide.
HCl is used to destroy the substances that unite the cells so that the cells spread easily and you can see one layer of cells on your slide.
follow this procedure.. 1.Collect two onion root tips and trim so that you have about 2 mm of the very tip. 2.Soak the tips in a few drops of 1 M HCl in a watch glass for about 10 min. 3.Transfer the tips to a few drops of acetocarmine stain for about 15 min. 4.Transfer one tip to a few drops of water on a slide. Lower a cover slip onto the tip and press it down gently so that the tip squashes out to about 7 mm (1/3 inch) around. 5.Look for mitoses under high power (X40) or under oil immersion (X100) (If this fails for any reason try your other root tip)