Why won't my power windows work in my Toyota Highlander?

FIRST - check the child-proof window lock setting to be certain that it isn't set in the "child-proof" position which prevents windows from being raised and lowered. SECOND - check the fuses and relays which control the operation of the electric windows. If none of these is the problem - you'll probably have to take it to a shop that can trace out an electrical problem for you.