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Q: Why you use 20 mins for autoclave sterilization?
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What is the definition of moist heat sterilization?

Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.


What is the Principle of autoclave in biotechnology?

An autoclave is piece of equipment that is usually used to sterilize objects and supplies by subjecting them to high pressure saturated steam at 121 °C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. The sterilization occurs by three mechanisms: temperature, pressure, and thermal oxidation. The high temperature will kill most microorganisms because they cannot tolerate the temperature - essentially it "cooks" them. Viruses, which may not be considered "organisms" are also broken down by the temperature and proteins can be denatured. The pressure will cause penetration of membrane walls of organisms, disrupting or breaking the walls, forcing the steam into them, and screwing up the osmotic pressure. Some biological materials will also experience thermal oxidation - they react with residual air present in the autoclave or with other oxidizers present in the autoclave. As the reactions occur, the biological materials are broken down or destroyed.


How long do you process redken color gels?

It depends on the volume of developer. 10 vol = 20 mins 20 vol = 30 mins 30 vol = 40 mins 40 vol = 45 min


How active is Mount Vesuvius and Mount Etna?

10-20 mins


How many mph is 15 minutes in 20 minutes?

You haven't mentioned a distance to go with the speed so it cannot be answered. However, if you're given miles in 15 mins, multiply by 4 and if 20 mins, multiply by 3 to get them per hour

Related questions

What is the adequate length of time for sterilization of a spay pack at 273 degrees in an autoclave?

20 mins


What are methods of soil sterilization?

Historically, a common method described in literature was "to sow salt." In practice this does not actually work, as salt is soluable and easily carried away by rain. Soil is partially sterlized through the use of herbicides, such as Agent Orange or Round Up. Covering patches of soil with black plastic is another method of sterilization that does not require adding chemicals. Herbicides are often sprayed along road ways and side walks to prevent the roots of trees and brush from cracking the concrete or pavement. Of course, covering land with asphalt is another effective "sterilization" technique. Finally, if one can afford the energy, running soil through an autoclave will sterilize it. This might be done for certain biology experiments in university laboratories. Soil is not typically autoclaved for regular agricultural use.


What is the definition of moist heat sterilization?

Dry-heat sterilization is generally a less complicated process than steam sterilization; it is, however, relatively slow and requires higher temperatures and/or longer exposure times. This is because of the fact that microbial lethality is lower with dry heat than that for steam at the same temperature.


What is autoclave and what is it used for?

pressure chamber used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C (249°F) for around 15-20 minutes depending on the size of the load and the contents


How many kwh does an electric oven use in 20 mins?

none, if it is unplugged


How minutes in 1 hour 20 mins?

1 hour = 60 minutes so 1 hr and 20 mins = 60+20 = 80 mins. Simple!


What is 1 minute and 42 seconds multiplied by 20?

20 mins + 840 seconds = which is 34 mins


What is the Principle of autoclave in biotechnology?

An autoclave is piece of equipment that is usually used to sterilize objects and supplies by subjecting them to high pressure saturated steam at 121 °C or more, typically for 15 to 20 minutes depending on the size of the load and the contents. The sterilization occurs by three mechanisms: temperature, pressure, and thermal oxidation. The high temperature will kill most microorganisms because they cannot tolerate the temperature - essentially it "cooks" them. Viruses, which may not be considered "organisms" are also broken down by the temperature and proteins can be denatured. The pressure will cause penetration of membrane walls of organisms, disrupting or breaking the walls, forcing the steam into them, and screwing up the osmotic pressure. Some biological materials will also experience thermal oxidation - they react with residual air present in the autoclave or with other oxidizers present in the autoclave. As the reactions occur, the biological materials are broken down or destroyed.


How long is 140 minutes?

60 mins=1 hour 60x2=120 mins=2 hours 60 can't go into 20 mins, so it's 2 hours and 20 minutes (2 hr & 20 mins)


Which animal goes to sleep for only 5 mins?

the giraffe sleeps for like 20 mins. there is no animal that sleeps for only 5 mins. SO... THE GIRAFFE SLEEPS FOR LIKE 20 MINS.


How long do you cook a 14 pound turkey in an autoclave?

you cook a 14 pound turkey for 4 hours, here it goes, 20 minutes per pound, which is 280 divide by 60 which is 4 hours and 20 minutes...and that would be without an autoclave. In an autoclave it looks like regardless of weight you'll run about a 45 minute sterilize cycle. (121C with 15 psig) I would use a liquid cycle, of course, to minimize/prevent boiling. The whole cycle would take about about an hour and 30 minutes. An autoclave safe container in which you can submerse the turkey in broth might help.


How do you clean a nasal speculum?

Easiest would be to boil it for about 20 minutes in water, or else autoclave it.