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"Heat will most definitely kill germs but freezing them won't do the trick- in the same way a frozen fish could spring back to life when put back into water. You could just be preserving them. Enzymes are at work in germs and these are denatured by extreme heat but cooling them might only be slowing them down. Freezing does prevent their multiplication if the temp is at 0°F or less. When thawed, the surviving organisms can multiply again."

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Q: Will freezing temperatures kill germs on a toothbrush in a sealed zip locked bag?
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Is there another word for locked?

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