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Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Mannitol salt agar or MSA is a commonly used growth medium in microbiology. It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective for Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria.
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
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Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
Mannitol salt agar inoculated with Micrococcus luteusshowing no fermentation of mannitol (pink medium). The colonies show a yellow pigment which is characteristic of M. luteus.
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
Mannitol is not a substrate for glycolisis
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
the salt content
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.