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No. First of all, there is a spelling error. It should be "lose" instead of "loose." You lose your keys, or you lose an opportunity. "Loose" is the opposite of "tight," as in a loose screw, a loose shoelace, or a loose woman.

Perhaps this sentence can help you remember the difference between the two: "If you lose weight your clothes will become too loose on you."

As for grammar, it should be "I" instead of "me": "You and I need to work hard to make sure we don't lose this opportunity."

An easy way to figure out whether to use "I" or "me" in sentences like this is to remove the other subject from the sentence. So it becomes, "Me need to work hard to make sure we don't lose this opportunity." Obviously, that does not make sense. But "I need to work hard to make sure we don't lose this opportunity" makes sense.

Another example might be: "Susan and me went to the store." Take out Susan, and it becomes, "Me went to the store." That's clearly wrong, so it must be, "Susan and Iwent to the store."

Here's one more: "Mom told Jason and I to go upstairs and put on an extra sweater." Take out Jason, and it becomes, "Mom told I to go upstairs and put on an extra sweater." Again, that's clearly wrong, so it must be, "Mom told Jason and me to go upstairs and put on an extra sweater." Take out Jason, and it becomes, "Mom told me to go upstairs and put on an extra sweater," which is clearly correct.

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Q: You and me need to work hard to make sure we don't loose this opportunity. Is this sentence structure correct?
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