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Franz Ziehl(Bacteriologist) andFrederick Neelson(pathologist) are the two scientists who discovered Ziehl-Neelson stain or acid-fast stain.Mycobacterium tuberculosis is an organism with high lipid conent in its cell wall because of which acid fast stain is used. 1.smearpreparation 2.flood with cabolfuchsin under continuous heating 3.wash with acid alcohol(20%hci:ethanol) 4.flood methelene blue.wash with water observe under microscope
Wiki User
∙ 14y ago
Wiki User
∙ 14y agoZ-N staining procedure is used to help differentiate between acid-fast and non-acid-fast microorganisms such as bacteria and fungi.
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What are some procedures and techniques used in biology?
Some procedures and techniques that are used in biology include dissecting, staining and sampling. Other techniques include cloning, testing and extraction.
What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
What is staining rack?
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
Why is safrinin used in gram staining?
as a couterstain
Which Stains are used for staining globin chains of hemoglobin?
eosin
What is the use of coplin jar?
A Coplin jar is used in laboratory settings to hold and process multiple microscope slides at the same time. It is commonly used for staining procedures, such as the Gram stain, where multiple slides need to be immersed in various staining reagents simultaneously.
What are some procedures and techniques used in biology?
Some procedures and techniques that are used in biology include dissecting, staining and sampling. Other techniques include cloning, testing and extraction.
Used for error detection?
Used for error detection
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Uses of staining rack?
used to hold the glass glides while staining them. (:
What is the difference between leishman staining and giemsa staining?
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
What is the function of staining jar?
There are several uses for a staining jar. In microscopy, it is used for staining tissues and cells for slides. After being stained with dyes or stains, the specimens can also be placed in the jar to look for certain aspects.
What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
What is staining rack?
Staining rack is used to hold many glass slides at a time. By putting slides on a staining rack, you can pour dye simultaneously and it will help to reduce extra dye or stain.
Why is safrinin used in gram staining?
as a couterstain
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
