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In order to reduce the possible volatile and long-term exposure effects of L-P CB, a reagent lacking phenol was developed to analyze inertial impaction samples. To date, approximately 20 000 individual spore-trap samples (Air-O-Cell cassette and Allergenco MK-5 Multi Trace Slide, Zefon International, St. Petersburg, Florida) have been analyzed using the new reagent, hereafter called Lacto-Cotton Blue (LCB). The recipe for Lacto-Cotton Blue mounting medium is: glycerol 250 mL (Sigma-Aldrich, St. Louis, Missouri), 85% lactic acid 100 mL (Sigma-Aldrich, St. Louis, Missouri), 3 mL of cotton blue stock, and de-ionized water 50 mL. A stock solution of Cotton Blue stain is made to add to the mounting medium: 85% lactic acid 99 mL, Aniline (Cotton) Blue crystals 1.0 g (Fisher Scientific, Los Angeles, California).
To make the Cotton Blue stock solution, first add cotton blue crystals to lactic acid while stirring vigorously on a stir-plate. Stir until cotton-blue crystals are dissolved. Next, vacuum filter the solution through a #50 Whatmann 90 mm filter disc (Fisher Scientific, Los Angeles, California) in a Büchner porcelain funnel. Cover the orifice of the porcelain funnel to insure airborne particulate matter cannot enter the reagent mixture. After filtration has occurred, check the clarity of the stock dye solution. Place one drop of the dye on a clean microslide with a 22 × 22 mm cover slip and examine at 400×. Particles of dye should be approximately 2 μm or less in diameter. If large clumps of dye are observed, repeat vacuum filtration of the dye solution until the appropriate clarity is obtained.
The next step is to make the LCB mounting medium. First, mix the water, lactic acid, and glycerine (in that order) for one hour on a stir-plate. Once the solution is homogenous, add three mL of Cotton Blue stock solution to the water, lactic acid, and glycerine solution. Stir the entire mixture for an additional hour. Cover the flask with laboratory film (Parafilm, Fisher Scientific, Los Angeles, California) while the mixture is stirring to insure against airborne contamination.
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How do you prepare alcian blue pH 1.0?
To prepare alcian blue pH 1.0 solution, dissolve alcian blue powder in an acidic solution with a pH of 1.0, such as hydrochloric acid or formic acid. Adjust the pH of the solution using a pH meter or indicator strips to ensure it reaches the desired pH before use.
How do you prepare thymol blue indicator?
Bromothymol Blue (BTB)Bromothymol blue is an indicator, a substance that changes color as the pH of a solution changes. Bromothymol blue is yellow in acidic solutions and blue in basic solutions.BTB can be prepared by mixing 0.1 g (a pinch) of bromothymol blue powder in 10 mL of a 4% solution of sodium hydroxide.Add 20 mL of alcohol and dilute to 1 L with distilled water.The solution should be deep blue.If it is green, add sodium hydroxide solution drop by drop until the solution turns blue.Bromothymol blue can be purchased from scientific supply houses.CAUTION: Sodium hydroxide is corrosive; the BTB solution should be prepared in a laboratory. BTB stains hands and clothes. Have students wear goggles and follow safety precautions when using BTB solution.
How do you prepare bicarbonate indicator?
To prepare a bicarbonate indicator, dissolve bromothymol blue powder (0.1g) in distilled water (100mL) to make a 0.1% solution. Adjust the pH slightly acidic by adding a few drops of dilute hydrochloric acid. The solution will turn yellow. It will act as an indicator turning blue in the presence of a base, indicating the presence of bicarbonate ions.
Which white powder turns blue-black in iodine solution?
Starch powder turns blue-black in iodine solution due to the formation of a starch-iodine complex.
What is the composition and purpose of lactophenol cotton blues?
Lactophenol cotton blue is a mixture of lactophenol, cotton blue, and glycerol. It is commonly used in microbiology to prepare and preserve fungal samples for microscopic examination. The lactophenol helps to kill and preserve the fungal cells, while cotton blue stains the fungal structures for better visualization under a microscope.
Which white powder turns orange in benedicts solution?
The white powder that turns orange in Benedict's solution is glucose. Benedict's reagent contains copper ions which can be reduced by glucose, resulting in a color change from blue to orange-red.
How does white dextrose powder turn a blue color while adding water?
White dextrose powder turns blue when added to water due to a phenomenon called Tyndall effect, where scattering of light by small particles in the solution makes it appear blue. The water refracts and scatters light differently based on the size of the dextrose particles, causing the perception of a blue color in the solution.
Why does the copper sulphate powder turn blue and gets hot when add water to it?
When water is added to copper sulfate powder, it dissolves to form a solution. This dissolution is an exothermic process, meaning it releases heat, causing the solution to become warm. The blue color of the solution is due to the copper ions in the copper sulfate forming a blue complex with water molecules.
Can cotton be blue?
Yes, cotton can be dyed blue.
What are facts about powder blue surgeon fish?
they are blue, powder blue to be precise.
What are the steps for conducting starch test on given sample of rice grain?
Crush the rice grain into a powder. Add a few drops of iodine solution to the powder. If the iodine solution turns blue-black, it indicates the presence of starch in the rice grain.
Can copper chloride react with sulfuric acid to produce blue salt solution?
Yes, when copper chloride reacts with sulfuric acid, it can produce a solution containing copper sulfate, which is a blue salt. This reaction is often used to prepare copper sulfate solutions for various applications.
