40x
Start with the lowest magnification objective lens, typically 4x or 5x, as it provides a wider field of view and makes it easier to locate the specimen.
The total magnification of a light microscope with a 40x objective lens is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece lens. Assuming a standard eyepiece magnification of 10x, the total magnification would be 400x (40x objective lens * 10x eyepiece lens = 400x total magnification).
The lowest magnification objective lens, such as the 4x lens, typically provides the largest field of view because it allows more of the specimen to be visible at once. This is useful for observing larger structures or getting an overall view of a specimen before zooming in with higher magnification lenses.
objective lens
There is a relationship between the power of an objective lens and its field of view. As the power of the objective lens increases, the size of its field of view decreases
40x
The field of view of the oil immersion 100x lens would be 0.4 mm. This is because the field of view decreases as the magnification increases.
The lens with the greatest (positive) curvature. The lens with the highest diopter.
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
The total magnification of a microscope is calculated by multiplying the power of the objective lens by the power of the eyepiece lens. Given a total magnification of 200x and an eyepiece lens power of 10x, the power of the objective lens would be 200x/10x = 20x.
You use the 3 objective lenses of a compound microscope to switch powers. There's LOW, MEDIUM, and HIGH power. With LOW power, you can magnify what you're looking at. With HIGH power, you can see things that you can't see with a naked eye.
The scanning electron microscope (SEM) has the greatest depth of field compared to other objective lenses in optical microscopes. This is because the SEM uses a focused beam of electrons to create an image, allowing for a larger range of depths to be in focus simultaneously.
The ocular is the upper lens and objective is the lower lens
Field diameter is calculated by measuring the distance across the field of view of a microscope, then dividing that measurement by the magnification of the objective lens being used. This gives you the field diameter in micrometers.
Start with the lowest magnification objective lens, typically 4x or 5x, as it provides a wider field of view and makes it easier to locate the specimen.
low power objective lens