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capsule
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India ink can give the appearance of a negative stain when used in a wet mount what is the base for this?
India ink contain carbon particles that are too large to penetrate the cell wall, therefore staining the background instead of the cell.
Why is the technique called negative staining?
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What is the purpose of the heat during the acid fast staining procedure?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What is the color of the capsule in the capsule stain?
When a stain, such as an acid dye, cannot penetrate the outer layers of a microbe, the cell will appear transparent on a colored background. This stain is called a negative or background stain. It is performed by mixing the dye with a suspension of bacteria on a slide and spreading the mixture into a thin layer for viewing. The capsule is a structure surrounding the cell wall that certain bacteria can produce. The ability to form a capsule is genetically and environmentally controlled. Only those microbes with the genes for capsule production have the potential to manufacture this polysaccharide (or polypeptide) surface layer. Special nutrients or other growth factors often are necessary for the genes to be expressed. The role of the capsule is primarily for protection of the bacteria. For example, the capsule affords a seal against dehydration. Many capsules repel white blood cells and thus allow pathogenic invading bacteria to elude one of the primary host defenses. Capsules are not readily stained and therefore are visualized by negative stain techniques. The organisms are prepared as a smear in the presence of an acid dye and allowed to air dry because heat will cause the capsule to shrink. Our procedure will combine a negative stain (which colors the background) and a simple stain to color the bacterial cell. The capsule appears as a colorless layer between the bacterium and the background.
India ink can give the appearance of a negative stain when used in a wet mount what is the base for this?
India ink contain carbon particles that are too large to penetrate the cell wall, therefore staining the background instead of the cell.
The background of a cell is called the?
cell background
Why is the technique called negative staining?
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
What is the purpose of the heat during the staining procedures?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Why must heat be used with application of the primary stain during acid fast staining?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Is coffee a true biological stain or just a ompound capable of imparting color?
It is simply a compound capable of imparting color. A biological stain refers to a compound that changes the color of features of a cell such as cell walls or the nucleus of a cell and helps to view them more clearly. A coffee stain does not do that.
What is the purpose of the heat during the acid fast staining procedure?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
How do you change the background color in a table cell?
Your on your own.
What is the background of a cell called in Excel?
Fill Color
What is the color of the capsule in the capsule stain?
When a stain, such as an acid dye, cannot penetrate the outer layers of a microbe, the cell will appear transparent on a colored background. This stain is called a negative or background stain. It is performed by mixing the dye with a suspension of bacteria on a slide and spreading the mixture into a thin layer for viewing. The capsule is a structure surrounding the cell wall that certain bacteria can produce. The ability to form a capsule is genetically and environmentally controlled. Only those microbes with the genes for capsule production have the potential to manufacture this polysaccharide (or polypeptide) surface layer. Special nutrients or other growth factors often are necessary for the genes to be expressed. The role of the capsule is primarily for protection of the bacteria. For example, the capsule affords a seal against dehydration. Many capsules repel white blood cells and thus allow pathogenic invading bacteria to elude one of the primary host defenses. Capsules are not readily stained and therefore are visualized by negative stain techniques. The organisms are prepared as a smear in the presence of an acid dye and allowed to air dry because heat will cause the capsule to shrink. Our procedure will combine a negative stain (which colors the background) and a simple stain to color the bacterial cell. The capsule appears as a colorless layer between the bacterium and the background.
What color are bacterial endospores after a gram stain is performed?
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
What color is a nucleus of a plan cell?
It does not have a color. If you look at it under a microscope, you can't see it unless you put a stain on it.
