0
Acidic
Wiki User
∙ 10y ago
Add your answer:
Earn +20 pts
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
What is carbolfuchsin?
This compound is basic.
What color are non acid fast organisms after staining?
Acid-fast organisms are characterized by wax-like cell walls.Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique.1. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. (violet or purple).2. This is further assisted by the addition of heat.3. The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms.4. The decolorized non-acid-fast cells then take up the counterstain. (safranin- red).Gram positive bacteria will stain purple.Gram negative bacteria will stain red/pink.
What is the difference between Wright stain and Giemsa stain?
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
What is carbolfuchsin's pH?
This compound is basic.
Can use carbolfuchsin as a counterstain?
The carbolfuchsin is a differential stain. It is an acid-fast stain which distinguishes organisms with waxy cell walls with the use of acid alcohol.
Does carbolfuchsin stain acid fast negative cells?
Yes, carbolfuchsin can stain acid-fast negative cells. This red dye can easily get into their thin cell wall lipids due to its solubility.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
What is the pH of buffer in the romanowsky stain?
6.8
What is carbolfuchsin?
This compound is basic.
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
# The pH will determine if the bacteria will have a particular charge. If the chromophore is a positive ion like the methylene blue in the equation shown in the reading, the stain is considered a basic stain; if it is a negative ion, it is an acidic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the negative charges of the bacterial cell.
How would the pH of the stain methylene blue affect the staining of bacteria?
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
If there is higher pH is the liquid denser?
no. its not like that pH usually have no relation or direct correlation with the density
Should a wood stain show brush lines?
No. If it's a penetrating stain, it's on too thick and the excess needs to be wiped off prior to drying. If it's a solid stain, you need to get a better quality brush or either move your work out of the direct sun or wait until it's not in the direct sun if it can't be moved.
What is an acid-fast pathogen?
An acid-fast pathogen is a bacteria that is harmful to humans. They have cell walls that contain mycolic acid which is a lipid. Common Gram type staining techniques wont work with these cells. A special stain carbolfuchsin is used to penetrate the wall.After staining you wash with acid alcohol if the stain remains it is acid fast if it washes out it is non-acid fast.Mycobacterium tuberculosis is a well known acid-fast pathogen
