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Some cells produce gram-variable results.

Some cells produce no results.

The age of the culture can influence the results.

It has limited usage in environmental Biology and is not as good as molecular techniques.

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Is the acid fast staining result positive or negative for the sample?

The acid-fast staining result for the sample is positive.


What staining procedure will be used to observe bacteria in the sputum sample?

A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.


Why are specimens are to be stained suspended in sterile water?

Specimens are stained in sterile water to prevent contamination of the sample, which could affect the staining process and lead to inaccurate results. Staining in sterile water helps maintain the integrity of the sample and ensures that the staining procedure is carried out under controlled conditions.


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There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.


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Staining affinity refers to the ability of a particular stain to bind specifically to certain components within a sample, such as cells or tissues, leading to their visualization under a microscope. Different stains have varying affinities for different structures, such as cell nuclei or cytoplasm, allowing for selective staining and highlighting of specific features in a sample. This characteristic is crucial for achieving clear and accurate microscopic images in biological and histological studies.

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