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Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?

smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.


What happens when you prepare a smear from a agar plate or slant without first placing water on slide?

If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.


What would be the result of overheating the bacterial smear?

Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.


Reasons why a smear from a gram-positive control showed a gram-negative result?

Possible reasons for a gram-positive control smear showing a gram-negative result could include errors in the staining process, contamination of the specimen with gram-negative bacteria, or misidentification of the control as a gram-positive organism when it is actually gram-negative. Additional troubleshooting and repeat testing may be needed to confirm the result.


Why do you air-dry a smear?

Air-drying a smear helps to fix the cells onto the slide, preventing any loss or distortion during further processing steps like staining or examination under a microscope. It also helps to evaporate any excess water, improving the visualization of the cells.

Related Questions

When was the first pap smear done in Australia?

1940


How do you know if you are going to have your period for the first time?

Well youll see a white smear in your underwear. And you may have cramps on the day before your period.


Does getting for the first time hurt?

no. well it didnt for me. i was 13 when i first was fingered and it was not painful. as long as u are either wet or you have lube you will be fine. and make sure they dont start with like three fingers s6tart slow


Can a pap smear stop you from conceiving?

A pap smear does not affect your fertility. You are as likely to get pregnant after a pap smear as you are before a pap smear.


Who were the characters in the inn of lost time?

three sons ZENTA tokubei six fingered girl male innkeeper


Do one have to go for a smear after a hysterectomy?

Unless your cervix was removed at the time of your hysterectomy then YES as the purpose of the smear is to find cancerous cervical cells before cancer sets in.


What can be observed on a plaque smear wet mount?

difference in plaque smear and yeast smear


When is the best time in your cycle to have a pap smear?

Getting a pap smear right between periods is easiest and most comfortable. You can get a pap smear anytime you're not bleeding heavily, though.


What is the abbreviation for papanicolaou smear?

Pap smear.


What is the past tense of smear?

The past tense of smear is smeared.


What is a pap smear urine test used for?

There is no "pap smear urine test." A urine test may be done at the time of the pap smear to detect other conditions. Talk with your heatlh care provider to find out what tests were done during your exam.


What is the latin name for the leaf-fingered gecko?

The latin name for leaf-fingered gecko is Phyllodactylus lanei ( At least that is what I have learned.)