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There are several factors that affect enzyme itself.

1. Temperature

As the shape specificity of the enzyme's active site is vital for any enzyme reaction, therefore an optimal temperature is important for enzymes. Enzymes will be inactive under low temperatures since it has too low kinetic energy to start the reaction or quickens the reaction. Alternatively, enzyme's structure and the shape of active site will changed in high temperature. Eventually, enzymes are denature and never can be used again.

2. pH value

As mentioned, the structure of enzyme is highly sensitive to the external environment, therefore pH value of the environment also has an effect on enzymes. Again, the shape of enzymes changed outside the range of pH value that suits particular enzymes. For instance, the optimum pH value for enzymes in stomach is around pH 2 whereas most are pH 7.

3. Presence of inhibitor

There are 2 types of inhibitor, one is non-competition inhibitor. These inhibitors will bind to the allosteric site (alternative site) of the enzymes that alters the shape of active site, therefore preventing the substrate from binding the enzymes and having reactions.

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