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Answering "Experiment on the effect of the PH of enzymatic reaction using hydrogen peroxide and extract of spinach leaf?"

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Q: Experiment on the effect of the PH of enzymatic reaction using hydrogen peroxide and extract of spinach leaf?
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What is the substrate of catalase?

A substrate is a substance in which an enzyme reacts. The substrate for catalase would be hydrogen peroxide otherwise known as H2O2.


What experiment shows how pH affects enzymes?

Equipment: - Hydrogen Peroxide - Catalase - Equally sized paper slips (a small rectangle, 2 cm X 1 cm) - Tweezers - Beaker - Stopwatch -pH testing strips -Acids (lemon juice, vinegar, etc.) -Bases (milk, baking soda, ammonia etc.) For the control, take one of the paper slips, using the tweezers, and dip it into the catalase, then place the catalase covered slip at the bottom of a beaker filled about halfway with hydrogen peroxide. You cannot simply drop the slip into the beaker, you must stick the tweezers into the beaker so that they reach the bottom, and then release. When the catalase contacts the hydrogen peroxide the hydrogen peroxide will release oxygen as a reaction, these oxygen bubbles in the liquid will push the paper to the top. Start the stop watch precisely when the strip enters the hydrogen peroxide, to measure the rate of reaction, and stop it again when the slip reaches the top. And finally for the affect of pH on the enzyme, simply change the pH of the hydrogen peroxide adding an acid, or a base. Then, just rerun the experiment and it will be evident that the pH affects the rate of reaction. The pH of hydrogen peroxide is roughly 2, or in that region, so adding a base will speed up the reaction, because the pH optimum of catalase is around 7.


What is the saturation point in an enzymatic reaction called?

half


Is 2H2O O2 synthetic or degradative reaction?

An example of spontaneous decomposition is that of hydrogen peroxide, which will slowly decompose into water and oxygen: : 2H2O2 → 2H2O + O2 thus it would be a degradative reaction


Why when adding catalase to sucrose not makes it bubble?

Catalase is an enzyme that is substrate-specific, meaning that it has a particular reaction that it will catalyze (to speed up a reaction). Hydrogen peroxide (H2O2) is the specific substrate reactant with which catalase will react (in a degradation reaction). This is the degradation (breaking down of) reaction. 2H2O2 yields 2H2O+O2 O2 is oxygen which is indicated by the rising of bubbles upon reaction between the hydrogen peroxide and the catalase. Sucrose is not the specific substrate assigned to the catalase enzyme, therefore they will not react together in a degradation reaction, hence the lack of oxygen release (lack of bubbles). I hope this was helpful to you. God bless and Jesus loves you.

Related questions

Why does hydrogen peroxide foam with potatoes?

Catalase is an enzyme that is present in potatoes that catalyzes the decomposition of the hydrogen peroxide into H20 (water) and O2 (oxygen). This oxygen that is being formed in the enzymatic reaction is the cause for the bubbles that you see forming.


Detail of reaction between sodium sulphite and hydrogen peroxide?

reaction betwen sodiumbisulphite and hydrogen peroxide


What happens when Alka-Seltzer is mixed with Hydrogen Peroxide?

having just done this experiment for my chemistry lab, i found that adding it to hydrogen peroxide produces a similar reaction as adding it to water. it dissolves almost as fast.


What is the Reaction of sand in hydrogen peroxide?

No reaction will occur.


What kind of reaction is made between hydrogen peroxide and yeast?

Hydrogen Peroxide decomposes in oxygen gas and water.


Why does liver and hydrogen peroxide exothermic?

Hydrogen peroxide is an oxidising agent, and oxidation is an exothermic reaction. The liver is being oxidised.


What chemical reaction degrades hydrogen peroxide in the presence of catalase?

Catalase is the endozyme that then degrades hydrogen peroxide into water and oxygen


When hydrogen peroxide is poured on a cut on a persons skin the hydrogen peroxide breaks down into water and oxygen gas what type of change is this?

This is a chemical reaction. A decomposition reaction.


What does hydrogen peroxide and sugar do?

The reaction of common household hydrogen peroxide is rather boring. But pure H2O2 (hydrogen peroxide) mixed with sugar which is C12H22O11 results in the production of H2O and CO2.


What effect does hydrogen peroxide have on potato and does it differ for each type of potato?

The hydrogen peroxide foams.


Is there a reaction between calcium carbonate and hydrogen peroxide?

yes


Is mixing hydrogen peroxide and sugar a chemical reaction?

yes